| Effect of antibiotics on development in vitro of hamster pronucleate ova | |
| Zhou H1,3; McKiernan SH2,3; Ji W*1,3; Bavister BD2,3,4; wji@mail.kiz.ac.cn | |
| 2000 | |
| 发表期刊 | THERIOGENOLOGY
 |
| ISSN | 0093-691X |
| 卷号 | 54期号:7页码:999-1006 |
| 摘要 | Antibiotics are commonly added to embryo culture media, but effects on embryo development have not been examined thoroughly. Hamster ova were used to investigate whether penicillin, streptomycin or gentamicin affect embryo development in vitro. Ova were collected 10 h post activation by spermatozoa in vivo and cultured in five treatments: 1) CONTROL: chemically-defined medium HECM-9 with no antibiotics; 2) HECM-9 with 100 IU/mL penicillin; 3) HECM-9 with 50 microg/mL streptomycin; 4) HECM-9 with 10 microg/mL gentamicin and 5) HECM-9 with both 100 IU/mL penicillin and 50 microg/mL streptomycin. Individually, penicillin, streptomycin and gentamicin did not affect embryo development to the 8-cell stage at 58 h post oocyte activation, or morula/blastocyst stages, or blastocysts alone at 82 h post activation. However, when penicillin and streptomycin were both present in the culture medium the percentages of 8-cell embryos at 58 h and blastocysts at 82 h were significantly lower than the control. No antibiotic treatment improved hamster embryo development in vitro. We caution against the use of penicillin and streptomycin together for hamster embryo culture, and show that it is not necessary to include any antibiotics in embryo culture media for up to 72 h if proper sterile technique is used with an oil overlay. |
| 关键词 | Antibiotics Penicillin Streptomycin Gentamicin Blastocyst Development |
| 资助者 | This work was supported by the NICHD, National Cooperative Program on Non-Human In Vitro Fertilization and Embryo Development (grant number HD22023); the Fogarty International Center, NM (grant number TW 00840); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 39970117. ; This work was supported by the NICHD, National Cooperative Program on Non-Human In Vitro Fertilization and Embryo Development (grant number HD22023); the Fogarty International Center, NM (grant number TW 00840); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 39970117. ; This work was supported by the NICHD, National Cooperative Program on Non-Human In Vitro Fertilization and Embryo Development (grant number HD22023); the Fogarty International Center, NM (grant number TW 00840); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 39970117. ; This work was supported by the NICHD, National Cooperative Program on Non-Human In Vitro Fertilization and Embryo Development (grant number HD22023); the Fogarty International Center, NM (grant number TW 00840); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 39970117. |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | This work was supported by the NICHD, National Cooperative Program on Non-Human In Vitro Fertilization and Embryo Development (grant number HD22023); the Fogarty International Center, NM (grant number TW 00840); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 39970117. ; This work was supported by the NICHD, National Cooperative Program on Non-Human In Vitro Fertilization and Embryo Development (grant number HD22023); the Fogarty International Center, NM (grant number TW 00840); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 39970117. ; This work was supported by the NICHD, National Cooperative Program on Non-Human In Vitro Fertilization and Embryo Development (grant number HD22023); the Fogarty International Center, NM (grant number TW 00840); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 39970117. ; This work was supported by the NICHD, National Cooperative Program on Non-Human In Vitro Fertilization and Embryo Development (grant number HD22023); the Fogarty International Center, NM (grant number TW 00840); the Chinese Academy of Sciences KZ952-Jl-109; the Yunnan Nature Sciences Foundation 98C092M; and the Chinese National Sciences Foundation 39970117. |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/5545 |
| 专题 | 生殖与发育生物学 |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 作者单位 | 1.Kunming Institute of Zoology, Chinese Academy of Science, Kunming, China 2.Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, Wisconsin USA 3.China-US Primate Biology Laboratory, Kunming, China 4.Present address: Dept. of Biological Sciences, University of New Orleans, and Audubon Center for Research of Endangered Species, New Orleans, Louisiana, USA. |
| 推荐引用方式 GB/T 7714 | Zhou H,McKiernan SH,Ji W*,et al. Effect of antibiotics on development in vitro of hamster pronucleate ova[J]. THERIOGENOLOGY,2000,54(7):999-1006. |
| APA | Zhou H,McKiernan SH,Ji W*,Bavister BD,&wji@mail.kiz.ac.cn.(2000).Effect of antibiotics on development in vitro of hamster pronucleate ova.THERIOGENOLOGY,54(7),999-1006. |
| MLA | Zhou H,et al."Effect of antibiotics on development in vitro of hamster pronucleate ova".THERIOGENOLOGY 54.7(2000):999-1006. |
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| 200054999.pdf（545KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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