Vitrification of Yunnan Yellow Cattle oocytes: work in progress

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	Vitrification of Yunnan Yellow Cattle oocytes: work in progress
	Li XL 1; Su L 1; Li YH 1; Ji WZ*1; Dinnyes A 2; wji@mail.kiz.ac.cn
	2002
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	58 期号:7 页码:1253-1260
合作性质	其它
摘要	The objective of this study was to provide a simple cryopreservation method for oocytes from Yunnan Yellow Cattle and facilitate preservation efforts in this native Chinese breed, which is threatened by agricultural modernization. Cumulus-oocyte complexes (COCs) were collected from slaughterhouse ovaries and matured in vitro for 22-24 h, then selected for cryopreservation. Vitrification in open pulled straws (OPS) or in microdrops on a cooled metal surface (solid surface vitrification, SSV) was compared. The OPS vitrification solution consisted of 20% ethylene glycol (EG) and 20% DMSO. The SSV solution was a mixture of 35% EG, 5% polyvinyl-pyrrolidon (PVP) and 0.4 M trehalose. Vitrified and warmed oocytes were either fertilized in vitro or parthenogenetically activated. The rates of cleavage and development to blastocysts of fertilized oocytes following OPS versus SSV were not statistically different (38.3 and 12.5% versus 35.8 and 6.0%, respectively). The corresponding rates of parthenogenetic development to blastocysts were also not different (8.2 versus 3.5%, respectively). Development to blastocysts of non-vitrified controls following fertilization was significantly higher than that of the vitrified oocytes (22.6%, P < 0.05). These results demonstrate for the first time, that although both OPS and SSV procedures reduced embryonic development, Yunnan Yellow Cattle oocytes are capable of developing to blastocysts following cryopreservation.
关键词	Bovine Oocyte Cryopreservation Ops Ssv
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收录类别	SCI
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5551
专题	生殖与发育生物学
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Chinese Acad Sci, Kunming Inst Zool, Kunming 650223, Yunnan, Peoples R China 2.Hungarian Acad Sci, Res Grp Anim Breeding, H-2103 Godollo, Hungary
推荐引用方式 GB/T 7714	Li XL,Su L,Li YH,et al. Vitrification of Yunnan Yellow Cattle oocytes: work in progress[J]. THERIOGENOLOGY,2002,58(7):1253-1260.
APA	Li XL,Su L,Li YH,Ji WZ*,Dinnyes A,&wji@mail.kiz.ac.cn.(2002).Vitrification of Yunnan Yellow Cattle oocytes: work in progress.THERIOGENOLOGY,58(7),1253-1260.
MLA	Li XL,et al."Vitrification of Yunnan Yellow Cattle oocytes: work in progress".THERIOGENOLOGY 58.7(2002):1253-1260.

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2002581253.pdf（91KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文