| 17 beta-Estradiol and progesterone improve in-vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys | |
| Zheng P1,2; Si W1,2; Bavister BD2,3; Yang JF1,2; Ding CH1,2; Ji WZ*1,2; wji@mail.kiz.ac.cn | |
| 2003 | |
| 发表期刊 | HUMAN REPRODUCTION
 |
| ISSN | 0268-1161 |
| 卷号 | 18期号:10页码:2137-2144 |
| 合作性质 | 其它 |
| 摘要 | BACKGROUND: Effects of 17beta-estradiol and progesterone on rhesus monkey oocyte maturation in vitro were evaluated by embryo development subsequent to IVF. METHODS AND RESULTS: In experiment 1, immature cumulus-oocyte complexes collected from unstimulated adult females during the non-breeding season were matured in modified medium CMRL-1066 containing various combinations of gonadotrophins (FSH + LH), estradiol and/or progesterone. Formation of morulae and blastocysts was greatest in oocytes matured in medium containing estradiol and/or progesterone, with or without gonadotrophins (morula 38-46%, blastocyst 14-20%) than in control oocytes matured without estradiol or progesterone (morula 14%, blastocyst 0%). In experiment 2, cumulus-oocyte complexes from unstimulated prepubertal female monkeys were matured in medium with gonadotrophins, estradiol or progesterone. The best development to the morula stage was obtained with oocytes matured with gonadotrophins and estradiol or gonadotrophins and progesterone (43 and 25 morulae, respectively), while control oocytes matured with gonadotrophins but without steroid hormones gave the poorest morula developmental response (12%). However, there was no difference in blastocyst development across all groups (0-3%). CONCLUSIONS: These results demonstrate that during rhesus monkey oocyte maturation in vitro: (i) estradiol or progesterone can improve oocyte developmental competence; (ii) immature oocytes from prepubertal versus adult females have differential responses to challenge with estradiol or progesterone. |
| 关键词 | Embryo Development 17b-estradiol/oocyte Maturation In Vitro Progesterone Rhesus Monkey |
| 资助者 | This research was supported by the major state basic research development program G2000016108, and the National Institutes of Health, USA (grant no. HD22023 and Training Grant no. RO3- TW00840 from the Fogarty International Center). ; This research was supported by the major state basic research development program G2000016108, and the National Institutes of Health, USA (grant no. HD22023 and Training Grant no. RO3- TW00840 from the Fogarty International Center). ; This research was supported by the major state basic research development program G2000016108, and the National Institutes of Health, USA (grant no. HD22023 and Training Grant no. RO3- TW00840 from the Fogarty International Center). ; This research was supported by the major state basic research development program G2000016108, and the National Institutes of Health, USA (grant no. HD22023 and Training Grant no. RO3- TW00840 from the Fogarty International Center). |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | This research was supported by the major state basic research development program G2000016108, and the National Institutes of Health, USA (grant no. HD22023 and Training Grant no. RO3- TW00840 from the Fogarty International Center). ; This research was supported by the major state basic research development program G2000016108, and the National Institutes of Health, USA (grant no. HD22023 and Training Grant no. RO3- TW00840 from the Fogarty International Center). ; This research was supported by the major state basic research development program G2000016108, and the National Institutes of Health, USA (grant no. HD22023 and Training Grant no. RO3- TW00840 from the Fogarty International Center). ; This research was supported by the major state basic research development program G2000016108, and the National Institutes of Health, USA (grant no. HD22023 and Training Grant no. RO3- TW00840 from the Fogarty International Center). |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/5561 |
| 专题 | 生殖与发育生物学 科研部门_哺乳动物胚胎发育（郑萍） |
| 通讯作者 | wji@mail.kiz.ac.cn |
| 作者单位 | 1.Department of Primate Biology,Kunming Institute of Zoology, Chinese Academy of Sciences 2.China–US Primate Biology Laboratory, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiao Chang Dong Lu, Kunming 650223, China 3.Department of Biological Sciences, University of New Orleans, and Audubon Center for Research of Endangered Species, New Orleans, Louisiana, USA |
| 推荐引用方式 GB/T 7714 | Zheng P,Si W,Bavister BD,et al. 17 beta-Estradiol and progesterone improve in-vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys[J]. HUMAN REPRODUCTION,2003,18(10):2137-2144. |
| APA | Zheng P.,Si W.,Bavister BD.,Yang JF.,Ding CH.,...&wji@mail.kiz.ac.cn.(2003).17 beta-Estradiol and progesterone improve in-vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys.HUMAN REPRODUCTION,18(10),2137-2144. |
| MLA | Zheng P,et al."17 beta-Estradiol and progesterone improve in-vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys".HUMAN REPRODUCTION 18.10(2003):2137-2144. |
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| 2003182137.pdf（86KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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