Serum-free culture of rhesus monkey embryonic stem cells

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	Serum-free culture of rhesus monkey embryonic stem cells
	Pei Y 1,2; Ma J 1; Zhang X 1,2; Ji W*1; wji@mail.kiz.ac.cn
	2003
发表期刊	ARCHIVES OF ANDROLOGY
ISSN	0148-5016
卷号	49 期号:5 页码:331-342
合作性质	其它
摘要	Previous studies have shown that the maintenance and proliferation of undifferentiated rhesus monkey embryonic stem (rES) cells requires medium supplemented with fetal bovine serum (FBS). Due to the uncharacterized composition and variation in serum nature, the present study aimed to replace the serum-containing medium with a serum-free medium in the rES cell culture. The results showed that after the initial 48-h culture in the routinely used serum-containing medium, rES cells can grow and proliferate for a prolonged period in the serum-free medium composed of DMEM supplemented with a cocktail of BSA, IGF-1, TGF-alpha, bFGF, aFGF, estradiol, and progesterone. rES cells cultured in the serum-free medium maintained high level of alkaline phosphatase activity and OCT4 level. There was no indication of differentiation as judged by the marker gene expression of all three embryonic germ layers and trophoblast. In addition, serum-free culture would not affect the passage capacity and differentiation potential of rES cells. This work will facilitate the future study of induced differentiation of rES cells and other applications.
关键词	Culture Embryonic Stem Cell Rhesus Monkey Serum-free Medium
资助者	This work was supported by the Major State Research Development Program (2001CB509905). ; This work was supported by the Major State Research Development Program (2001CB509905). ; This work was supported by the Major State Research Development Program (2001CB509905). ; This work was supported by the Major State Research Development Program (2001CB509905).
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收录类别	SCI
语种	英语
资助者	This work was supported by the Major State Research Development Program (2001CB509905). ; This work was supported by the Major State Research Development Program (2001CB509905). ; This work was supported by the Major State Research Development Program (2001CB509905). ; This work was supported by the Major State Research Development Program (2001CB509905).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5563
专题	生殖与发育生物学
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, Yunnan, China 2.Graduate School, the Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	Pei Y,Ma J,Zhang X,et al. Serum-free culture of rhesus monkey embryonic stem cells[J]. ARCHIVES OF ANDROLOGY,2003,49(5):331-342.
APA	Pei Y,Ma J,Zhang X,Ji W*,&wji@mail.kiz.ac.cn.(2003).Serum-free culture of rhesus monkey embryonic stem cells.ARCHIVES OF ANDROLOGY,49(5),331-342.
MLA	Pei Y,et al."Serum-free culture of rhesus monkey embryonic stem cells".ARCHIVES OF ANDROLOGY 49.5(2003):331-342.

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