Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender

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	Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender
	Li YH 1,2,3; Cai KJ 1; Su L 1; Guan M 1; He XC 1; Wang H 1; Kovacs A 4; Ji WZ*1; wji@mail.kiz.ac.cn
	2005
发表期刊	ASIAN JOURNAL OF ANDROLOGY
ISSN	1008-682X
卷号	7 期号:2 页码:139-144
合作性质	其它
摘要	AIM: To establish a method for cynomolgus monkey sperm cryopreservation in a chemically defined extender. METHODS: Semen samples were collected by electro-ejaculation from four sexually mature male cynomolgus monkeys. The spermatozoa were frozen in straws by liquid nitrogen vapor using egg-yolk-free Tes-Tris mTTE synthetic extender and glycerol as cryoprotectant. The effects of glycerol concentration (1 %, 3 %, 5 %, 10 % and 15 % [v/v]) and its equilibration time (10 min, 30 min, 60 min and 90 min) on post-thaw spermatozoa were examined by sperm motility and sperm head membrane integrity. RESULTS: The post-thaw motility and head membrane integrity of spermatozoa were significantly higher (P0.05) for 5 % glycerol (42.95 +/- 2.55 and 50.39+/- 2.42, respectively) than those of the other groups (1%: 19.19 +/- 3.22 and 24.84 +/- 3.64; 3%: 34.23 +/- 3.43 and 41.37 +/- 3.42; 10%:15.68 +/- 2.36 and 21.39 +/- 3.14; 15%: 7.47 +/- 1.44 and 12.90 +/- 2.18). The parameters for 30 min equilibration(42.95 +/- 2.55 and 50.39 +/- 2.42) were better (P0.05) than those of the other groups (10 min: 31.33 +/- 3.06 and 38.98 +/- 3.31; 60 min: 32.49 +/- 3.86 and 40.01 +/- 4.18; 90 min: 31.16 +/- 3.66 and 38.30 +/- 3.78). Five percent glycerol and 30 min equilibration yielded the highest post-thaw sperm motility and head membrane integrity. CONCLUSION: Cynomolgus monkey spermatozoa can be successfully cryopreserved in a chemically defined extender, which is related to the concentration and the equilibration time of glycerol.
关键词	Cryopreservation Macaca Fascicularis Chemically Defined Extender Glycerol
资助者	This work was supported by grants from the Chinese Academy of Science’s KSCX 1-05-01, major state research development program G2000016108, Ministry of Science and Technology of China (2001 DEA 10009-09) and National Nature Sci- ence Foundation of China (No. 30370166). ; This work was supported by grants from the Chinese Academy of Science’s KSCX 1-05-01, major state research development program G2000016108, Ministry of Science and Technology of China (2001 DEA 10009-09) and National Nature Sci- ence Foundation of China (No. 30370166). ; This work was supported by grants from the Chinese Academy of Science’s KSCX 1-05-01, major state research development program G2000016108, Ministry of Science and Technology of China (2001 DEA 10009-09) and National Nature Sci- ence Foundation of China (No. 30370166). ; This work was supported by grants from the Chinese Academy of Science’s KSCX 1-05-01, major state research development program G2000016108, Ministry of Science and Technology of China (2001 DEA 10009-09) and National Nature Sci- ence Foundation of China (No. 30370166).
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收录类别	SCI
语种	英语
资助者	This work was supported by grants from the Chinese Academy of Science’s KSCX 1-05-01, major state research development program G2000016108, Ministry of Science and Technology of China (2001 DEA 10009-09) and National Nature Sci- ence Foundation of China (No. 30370166). ; This work was supported by grants from the Chinese Academy of Science’s KSCX 1-05-01, major state research development program G2000016108, Ministry of Science and Technology of China (2001 DEA 10009-09) and National Nature Sci- ence Foundation of China (No. 30370166). ; This work was supported by grants from the Chinese Academy of Science’s KSCX 1-05-01, major state research development program G2000016108, Ministry of Science and Technology of China (2001 DEA 10009-09) and National Nature Sci- ence Foundation of China (No. 30370166). ; This work was supported by grants from the Chinese Academy of Science’s KSCX 1-05-01, major state research development program G2000016108, Ministry of Science and Technology of China (2001 DEA 10009-09) and National Nature Sci- ence Foundation of China (No. 30370166).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5587
专题	生殖与发育生物学科研部门_哺乳动物胚胎发育（郑萍）
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 3.College of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan 650201, China 4.Research Institute for Animal Breeding and Nutrition, 2053 Herceghalom, Hungary
推荐引用方式 GB/T 7714	Li YH,Cai KJ,Su L,et al. Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender[J]. ASIAN JOURNAL OF ANDROLOGY,2005,7(2):139-144.
APA	Li YH.,Cai KJ.,Su L.,Guan M.,He XC.,...&wji@mail.kiz.ac.cn.(2005).Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender.ASIAN JOURNAL OF ANDROLOGY,7(2),139-144.
MLA	Li YH,et al."Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender".ASIAN JOURNAL OF ANDROLOGY 7.2(2005):139-144.

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