Effects of various extenders and permeating cryoprotectants on cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa

KIZ OpenIR > 生殖与发育生物学

	Effects of various extenders and permeating cryoprotectants on cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa
	Li YH 1,2,3; Cai KJ 1; Kovacs A 4; Ji WZ*1; wji@mail.kiz.ac.cn
	2005
发表期刊	JOURNAL OF ANDROLOGY
ISSN	0196-3635
卷号	26 期号:3 页码:387-395
合作性质	其它
摘要	The cryoprotective effects of 11 different extenders, TTE, DM, mDM, LG-DM, G-DM, TCG, TEST, TSM, Test-M, Test-H, and LM, on sperm cryopreservation of cynomolgus monkey (Macaca fascicularis) have been compared with glycerol as cryoprotectant. Sperm motility, plasma membrane, and acrosomal integrity were examined to evaluate frozen-thawed sperm function. The results showed that TTE, DM, mDM, LG-DM, G-DM, and TCG exhibited the best and similar protective efficiencies for cynomolgus monkey sperm cryopreservation in terms of sperm motility and plasma membrane integrity (P > .05). The acrosomal integrity for spermatozoa cryopreserved in TCG was statistically lower than that of TTE, DM, mDM, LG-DM, and G-DM (P < .05) but was significantly higher than that of TEST, TSM, Test-M, Test-H, and LM (P < .05). The postthaw sperm motility for 5 other extenders (TEST, TSM, Test-M, Test-H, and LM) did not exceed 30%, and the 3 sperm parameters evaluated for them were significantly lower than that of TTE, DM, mDM, LG-DM, G-DM, and TCG (P < .05). On the basis of these findings, 5 commonly used permeating cryoprotectants, glycerol, ethylene glycol, dimethyl sulfoxide, acetamide and propylene glycol have further been tested for their effectiveness on sperm cryopreservation in extenders of TTE, DM, mDM, LG-DM, G-DM, and TCG. The results showed that the sperm cryoprotective efficiencies of glycerol and ethylene glycol were similar and best among 5 permeating cryoprotectant treatments (P > .05). Dimethyl sulfoxide or acetamide resulted in average cryoprotection for cynomolgus monkey spermatozoa: poorer than glycerol or ethylene glycol but better than that of propylene glycol (P < .05). In addition, the action of permeating cryoprotectant appeared to be independent of extenders. The results in the present study demonstrate that 1) TTE, DM, mDM, LG-DM, G-DM, and TCG are excellent extenders and suitable for cynomolgus monkey sperm cryopreservation; 2) the mechanism of action of permeating cryoprotectants are not affected by extender composition; 3) ethylene glycol has a similar cryoprotective efficacy to glycerol that makes it a successful cryoprotectant for sperm cryopreservation in cynomolgus monkeys.
关键词	Sperm Freezing Motility Plasma Membrane Acro- Some Permeability Coefficient
学科领域	Andrology
资助者	Supported by grants from the Chinese Academy of Sciences KSCX 1- 05-01, major state research development program G2000016108, Minis- try of Science and Technology of China (2001 DEA 10009-09) and China National Science Foundation 30370166. ; Supported by grants from the Chinese Academy of Sciences KSCX 1- 05-01, major state research development program G2000016108, Minis- try of Science and Technology of China (2001 DEA 10009-09) and China National Science Foundation 30370166. ; Supported by grants from the Chinese Academy of Sciences KSCX 1- 05-01, major state research development program G2000016108, Minis- try of Science and Technology of China (2001 DEA 10009-09) and China National Science Foundation 30370166. ; Supported by grants from the Chinese Academy of Sciences KSCX 1- 05-01, major state research development program G2000016108, Minis- try of Science and Technology of China (2001 DEA 10009-09) and China National Science Foundation 30370166.
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收录类别	SCI
语种	英语
资助者	Supported by grants from the Chinese Academy of Sciences KSCX 1- 05-01, major state research development program G2000016108, Minis- try of Science and Technology of China (2001 DEA 10009-09) and China National Science Foundation 30370166. ; Supported by grants from the Chinese Academy of Sciences KSCX 1- 05-01, major state research development program G2000016108, Minis- try of Science and Technology of China (2001 DEA 10009-09) and China National Science Foundation 30370166. ; Supported by grants from the Chinese Academy of Sciences KSCX 1- 05-01, major state research development program G2000016108, Minis- try of Science and Technology of China (2001 DEA 10009-09) and China National Science Foundation 30370166. ; Supported by grants from the Chinese Academy of Sciences KSCX 1- 05-01, major state research development program G2000016108, Minis- try of Science and Technology of China (2001 DEA 10009-09) and China National Science Foundation 30370166.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5589
专题	生殖与发育生物学
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 2.Graduate School of the Chinese Academy of Sciences, Beijing, China 3.College of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China 4.Research Institute for Animal Breeding and Nutrition, Herceghalom, Gesztenyes, Hungary.
推荐引用方式 GB/T 7714	Li YH,Cai KJ,Kovacs A,et al. Effects of various extenders and permeating cryoprotectants on cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa[J]. JOURNAL OF ANDROLOGY,2005,26(3):387-395.
APA	Li YH,Cai KJ,Kovacs A,Ji WZ*,&wji@mail.kiz.ac.cn.(2005).Effects of various extenders and permeating cryoprotectants on cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa.JOURNAL OF ANDROLOGY,26(3),387-395.
MLA	Li YH,et al."Effects of various extenders and permeating cryoprotectants on cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa".JOURNAL OF ANDROLOGY 26.3(2005):387-395.

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