A comparative approach to somatic cell nuclear transfer in the rhesus monkey

KIZ OpenIR > 生殖与发育生物学

	A comparative approach to somatic cell nuclear transfer in the rhesus monkey
	Zhou Q1,2; Yang SH 2; Ding CH 2; He XC 2; Xie YH 2; Hildebrandt TB 3; Mitalipov SM 4; Tang XH 2; Wolf DP4; Ji WZ*2; qzhou@ioz.ac.cn ; wolfd@ohsu.edu ; wji@mail.kiz.ac.cn
	2006
发表期刊	HUMAN REPRODUCTION
ISSN	0268-1161
卷号	21 期号:10 页码:2564-2571
合作性质	其它
摘要	BACKGROUND: Despite the potential utility of primate somatic cell nuclear transfer (SCNT) to biomedical research and to the production of autologous embryonic stem (ES) cells for cell- or tissue-based therapy, a reliable method for SCNT is not yet available. Employing the rhesus monkey as a clinically relevant animal model, we have compared a conventional electrofusion method for SCNT with a one-step micromanipulation (OSM) method. METHODS: A prospective, randomized trial was conducted using only oocytes that were mature [metaphase II (MII)] at collection and a fibroblast-like cell line as nuclear donor cells (fetal fibroblasts). The embryos produced were characterized for in vitro developmental potential, cell number, karyotype and expression of nuclear mitotic apparatus (NuMA) and OCT-4. RESULTS: An in vitro blastocyst development rate of 24.4% was achieved with the OSM method, significantly higher than the 12.2% obtained following electrofusion. SCNT-produced embryos expressed normal karyotypes, cell numbers and NuMA and OCT-4 proteins in most cases. SCNT with male nuclear donor cells resulted in the production of male, SCNT blastocysts, eliminating the possibility of a parthenogenetic origin. Of the four fibroblast cell lines tested as nuclear donor cells, two supported the routine production of blastocysts following SCNT. CONCLUSIONS: The application of a modified SCNT technique (OSM) followed by embryo culture in hamster embryo culture medium-10 (HECM-10) allows, for the first time, the routine production of SCNT blastocysts, most of which appear normal by immunochemical, cytochemical and in vitro developmental criteria. These embryos will provide a resource for isolating ES cells and for studies of nuclear reprogramming by monkey cytoplasts.
关键词	Rhesus Monkey Somatic Cell Nuclei Transfer Therapeutic Cloning
资助者	This study was supported by the Chinese Academy of Sciences, KSCX1-05-02 and Western Talent Program; Major State Basic Development Program, 2004CCA01300 and 2006CB701500; and China National Science Foundation 30370166 and 30300176. Funding to pay the Open Access publication charges for this article was provided by the Knowledge Innovation Program of the Chinese Academy of Sciences, KSCXI-05. ; This study was supported by the Chinese Academy of Sciences, KSCX1-05-02 and Western Talent Program; Major State Basic Development Program, 2004CCA01300 and 2006CB701500; and China National Science Foundation 30370166 and 30300176. Funding to pay the Open Access publication charges for this article was provided by the Knowledge Innovation Program of the Chinese Academy of Sciences, KSCXI-05. ; This study was supported by the Chinese Academy of Sciences, KSCX1-05-02 and Western Talent Program; Major State Basic Development Program, 2004CCA01300 and 2006CB701500; and China National Science Foundation 30370166 and 30300176. Funding to pay the Open Access publication charges for this article was provided by the Knowledge Innovation Program of the Chinese Academy of Sciences, KSCXI-05. ; This study was supported by the Chinese Academy of Sciences, KSCX1-05-02 and Western Talent Program; Major State Basic Development Program, 2004CCA01300 and 2006CB701500; and China National Science Foundation 30370166 and 30300176. Funding to pay the Open Access publication charges for this article was provided by the Knowledge Innovation Program of the Chinese Academy of Sciences, KSCXI-05.
URL	查看原文
收录类别	SCI
语种	英语
资助者	This study was supported by the Chinese Academy of Sciences, KSCX1-05-02 and Western Talent Program; Major State Basic Development Program, 2004CCA01300 and 2006CB701500; and China National Science Foundation 30370166 and 30300176. Funding to pay the Open Access publication charges for this article was provided by the Knowledge Innovation Program of the Chinese Academy of Sciences, KSCXI-05. ; This study was supported by the Chinese Academy of Sciences, KSCX1-05-02 and Western Talent Program; Major State Basic Development Program, 2004CCA01300 and 2006CB701500; and China National Science Foundation 30370166 and 30300176. Funding to pay the Open Access publication charges for this article was provided by the Knowledge Innovation Program of the Chinese Academy of Sciences, KSCXI-05. ; This study was supported by the Chinese Academy of Sciences, KSCX1-05-02 and Western Talent Program; Major State Basic Development Program, 2004CCA01300 and 2006CB701500; and China National Science Foundation 30370166 and 30300176. Funding to pay the Open Access publication charges for this article was provided by the Knowledge Innovation Program of the Chinese Academy of Sciences, KSCXI-05. ; This study was supported by the Chinese Academy of Sciences, KSCX1-05-02 and Western Talent Program; Major State Basic Development Program, 2004CCA01300 and 2006CB701500; and China National Science Foundation 30370166 and 30300176. Funding to pay the Open Access publication charges for this article was provided by the Knowledge Innovation Program of the Chinese Academy of Sciences, KSCXI-05.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5591
专题	生殖与发育生物学科研部门_哺乳动物胚胎发育（郑萍）中国科学院昆明灵长类研究中心
通讯作者	qzhou@ioz.ac.cn; wolfd@ohsu.edu; wji@mail.kiz.ac.cn
作者单位	1.State Key Lab of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 2.Kunming Primate Research Center and Kunming Institute of Zoology, The Chinese Academy of Sciences, Yunnan, China 3.Institute for Zoo and Wildlife Research (IZW), Berlin, Germany 4.Oregon National Primate Research Center, Beaverton, OR, USA
推荐引用方式 GB/T 7714	Zhou Q*,Yang SH,Ding CH,et al. A comparative approach to somatic cell nuclear transfer in the rhesus monkey[J]. HUMAN REPRODUCTION,2006,21(10):2564-2571.
APA	Zhou Q*.,Yang SH.,Ding CH.,He XC.,Xie YH.,...&wji@mail.kiz.ac.cn.(2006).A comparative approach to somatic cell nuclear transfer in the rhesus monkey.HUMAN REPRODUCTION,21(10),2564-2571.
MLA	Zhou Q*,et al."A comparative approach to somatic cell nuclear transfer in the rhesus monkey".HUMAN REPRODUCTION 21.10(2006):2564-2571.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2006212564.pdf（259KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文