| Feeder layer- and serum-free culture of rhesus monkey embryonic stem cells | |
| Zhang XZ*1,2,3; Wang SF1,2; Yang SH1; Li TQ1,2; Ji SH1,2; Chen HW1,2; Li B1,2; Jin LF1,2; Xie YH1; Hu ZX1,2; Chi JX4; sleevexz@yahoo.com.cn | |
| 2006 | |
| 发表期刊 | REPRODUCTIVE BIOMEDICINE ONLINE
 |
| ISSN | 1472-6483 |
| 卷号 | 13期号:3页码:412-420 |
| 摘要 | The common culture system of rhesus monkey embryonic stem (rES) cells depends largely on feeder cells and serum, which limits the research and application of rES cells. This study reports a feeder layer-free and serum-free system for culture of rES cells. rES cells could be cultured through at least 22 passages on laminin in medium supplemented with serum replacement (SR), basic fibroblast growth factor (bFGF) and transforming growth factor beta1 (TGFbeta1), and maintained stable proliferation rates and normal karyotypes, while displaying all the embryonic stem cell characteristics including morphology, alkaline phosphatase (AKP), Oct-4, cell surface markers SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and formed cystic embryoid bodies in vitro. In addition, the studies showed that TGFbeta1, bFGF and laminin are necessary for maintaining the undifferentiated growth of rES cells in long-term culture. Moreover, withdrawal of TGFbeta1 increased the differentiation rate by decreasing the expression of integrins. Therefore, this system would provide a well-defined culture system for rES cells, and would facilitate research into self-renewal and differentiation mechanisms of rES cells. |
| 关键词 | Bfgf Embryonic Stem Cell Laminin Rhesus Monkey Embryonic Stem Cell Serum-free |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/5593 |
| 专题 | 生殖与发育生物学 中国科学院昆明灵长类研究中心 细胞与分子进化重点实验室 |
| 通讯作者 | sleevexz@yahoo.com.cn |
| 作者单位 | 1.Department of Reproduction and Development, Kunming Institute of Zoology and Kunming Primate Research Centre, Chinese Academy of Sciences, Kunming, Yunnan 2.Graduate School, Chinese Academy of Sciences, Beijing 3.College of Life Science, Shandong University of Technology, Zibo, Shandong 4.Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China |
| 推荐引用方式 GB/T 7714 | Zhang XZ*,Wang SF,Yang SH,et al. Feeder layer- and serum-free culture of rhesus monkey embryonic stem cells[J]. REPRODUCTIVE BIOMEDICINE ONLINE,2006,13(3):412-420. |
| APA | Zhang XZ*.,Wang SF.,Yang SH.,Li TQ.,Ji SH.,...&sleevexz@yahoo.com.cn.(2006).Feeder layer- and serum-free culture of rhesus monkey embryonic stem cells.REPRODUCTIVE BIOMEDICINE ONLINE,13(3),412-420. |
| MLA | Zhang XZ*,et al."Feeder layer- and serum-free culture of rhesus monkey embryonic stem cells".REPRODUCTIVE BIOMEDICINE ONLINE 13.3(2006):412-420. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 200613412.pdf（559KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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