The application of in vitro sperm competition test to evaluate the impact of ZP-derived peptides on fertilization capacity of cat sperm

KIZ OpenIR > 生殖与发育生物学

	The application of in vitro sperm competition test to evaluate the impact of ZP-derived peptides on fertilization capacity of cat sperm
	Niu YY 1,2,3; Greube A 1; Ji WZ 2; Jewgenow K*1; jewgenow@izw-berlin.de
	2006
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	66 期号:4 页码:989-995
摘要	The present study aimed to establish a sensitive in vitro assay to assess the binding capacity of cat spermatozoa. Cat oocytes and epididymal sperm cells were isolated from gonads and cultured for in vitro fertilization. Before fertilization, the sperm cells were incubated either in 10 mu M green dye Fluo-3-AM or 10 mu M orange dye CellTracker Orange CMTMR (Molecular Probes), respectively. After removing the dyes by washing, sperm cells stained with each dye were added to medium drops containing oocytes in various proportions and cultured for 16 h at 37 degrees C, 5% CO2. The oocytes were examined using fluorescence microscopy. Sperm bound to oocytes, and stained with different colors, were counted. When fresh epididymal sperm were mixed in at a specific proportion, the number of sperm bound to the zona pellucida (ZP) of oocytes reflected the proportion of differently colored sperm in the medium. This indicated that neither dye influenced the binding capacity of cat sperm. Mixing fresh and cryopreserved sperm, however, resulted in a higher number of fresh sperm bound to the oocyte surface in comparison to frozen-thawed sperm. Also, the pre-incubation of cat sperm cells with ZP derived peptide reduced the sperm binding capacity by 40%. In conclusion, the presented sperm competition assay allows assessment of fertilizing capacity of cat spermatozoa in vitro when a mixture of two different populations is used. The applied supravital fluorescence dyes do not affect motility and binding capacity of sperm cells and were clearly distinguishable under fluorescence microscopy. We demonstrate that the assay can be used to study the impact of sperm treatment, such as cryopreservation or pre-incubation in bioactive peptides, on fertilizing capacity.
关键词	Cats Immunocontraception Zona Pellucida Sperm Competition
资助者	This work received financial support from Max- Planck-Society and Chinese Academy of Sciences. ; This work received financial support from Max- Planck-Society and Chinese Academy of Sciences. ; This work received financial support from Max- Planck-Society and Chinese Academy of Sciences. ; This work received financial support from Max- Planck-Society and Chinese Academy of Sciences.
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收录类别	SCI
资助者	This work received financial support from Max- Planck-Society and Chinese Academy of Sciences. ; This work received financial support from Max- Planck-Society and Chinese Academy of Sciences. ; This work received financial support from Max- Planck-Society and Chinese Academy of Sciences. ; This work received financial support from Max- Planck-Society and Chinese Academy of Sciences.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5595
专题	生殖与发育生物学中国科学院昆明灵长类研究中心
通讯作者	jewgenow@izw-berlin.de
作者单位	1.Institute for Zoo and Wildlife Research, PF 601103, D-10252 Berlin, Germany 2.Kunming Primate Research Center and Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiao Chang Dong Lu, Kunming, Yunnan 650223, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Niu YY,Greube A,Ji WZ,et al. The application of in vitro sperm competition test to evaluate the impact of ZP-derived peptides on fertilization capacity of cat sperm[J]. THERIOGENOLOGY,2006,66(4):989-995.
APA	Niu YY,Greube A,Ji WZ,Jewgenow K*,&jewgenow@izw-berlin.de.(2006).The application of in vitro sperm competition test to evaluate the impact of ZP-derived peptides on fertilization capacity of cat sperm.THERIOGENOLOGY,66(4),989-995.
MLA	Niu YY,et al."The application of in vitro sperm competition test to evaluate the impact of ZP-derived peptides on fertilization capacity of cat sperm".THERIOGENOLOGY 66.4(2006):989-995.

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