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Comparative studies with six extenders for sperm cryopreservation in the cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta)
Li YH1,2,3; Cai KJ1; Li J1; Dinnyes A4; Ji WZ*1; wji@mail.kiz.ac.cn
2006
发表期刊AMERICAN JOURNAL OF PRIMATOLOGY
ISSN0275-2565
卷号68期号:1页码:39-49
摘要Ejaculated spermatozoa from cynomolgus monkeys and rhesus monkeys were frozen in straws with six different extenders (TTE, DM, mDM, LG-DM, G-DM, and TCG) containing glycerol. Sperm motility and head membrane and acrosomal integrity were evaluated after freezing and thawing, and the cryoprotective effects were compared among the extenders and the two species studied. The results showed that sperm motility and motility recovery with the six extenders were comparable for the cynomolgus and rhesus monkeys. There was no significant difference in sperm motility and head membrane integrity among the six extenders in either the cynomolgus or rhesus monkeys (P>0.05). However, a slightly but statistically lower percentage of acrosomal integrity was found with TCG in both species compared to the other extenders (P<0.05). These findings demonstrate that TTE, DM, mDM, LG-DM, G-DM, and TCG are equally suitable extenders for the cryopreservation of spermatozoa from cynomolgus and rhesus monkeys.
关键词Extender Cryopreservation Macaca Fascicularis Macaca Mulatta
资助者Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001 DEA10009-09; Contract grant sponsor: Major State Research Development Program; Contract grant number: G200016108; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KSCX1-05; Contract grant sponsor: China National Science Foundation; Contract grant number: 30370166; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Technological Collaboration; Contract grant number: TET-CHN10/03. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001 DEA10009-09; Contract grant sponsor: Major State Research Development Program; Contract grant number: G200016108; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KSCX1-05; Contract grant sponsor: China National Science Foundation; Contract grant number: 30370166; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Technological Collaboration; Contract grant number: TET-CHN10/03. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001 DEA10009-09; Contract grant sponsor: Major State Research Development Program; Contract grant number: G200016108; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KSCX1-05; Contract grant sponsor: China National Science Foundation; Contract grant number: 30370166; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Technological Collaboration; Contract grant number: TET-CHN10/03. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001 DEA10009-09; Contract grant sponsor: Major State Research Development Program; Contract grant number: G200016108; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KSCX1-05; Contract grant sponsor: China National Science Foundation; Contract grant number: 30370166; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Technological Collaboration; Contract grant number: TET-CHN10/03.
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语种英语
资助者Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001 DEA10009-09; Contract grant sponsor: Major State Research Development Program; Contract grant number: G200016108; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KSCX1-05; Contract grant sponsor: China National Science Foundation; Contract grant number: 30370166; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Technological Collaboration; Contract grant number: TET-CHN10/03. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001 DEA10009-09; Contract grant sponsor: Major State Research Development Program; Contract grant number: G200016108; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KSCX1-05; Contract grant sponsor: China National Science Foundation; Contract grant number: 30370166; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Technological Collaboration; Contract grant number: TET-CHN10/03. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001 DEA10009-09; Contract grant sponsor: Major State Research Development Program; Contract grant number: G200016108; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KSCX1-05; Contract grant sponsor: China National Science Foundation; Contract grant number: 30370166; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Technological Collaboration; Contract grant number: TET-CHN10/03. ; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001 DEA10009-09; Contract grant sponsor: Major State Research Development Program; Contract grant number: G200016108; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KSCX1-05; Contract grant sponsor: China National Science Foundation; Contract grant number: 30370166; Contract grant sponsor: Chinese-Hungarian Bilateral Scientific and Technological Collaboration; Contract grant number: TET-CHN10/03.
文献类型期刊论文
条目标识符http://ir.kiz.ac.cn/handle/152453/5603
专题生殖与发育生物学
通讯作者wji@mail.kiz.ac.cn
作者单位1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
2.Graduate School, Chinese Academy of Sciences, Beijing, China
3.College of Food Science and Technology, Yunnan Agricultural University, Kunming, China
4.Research Group on Applied Animal Genetics and Biotechnology, Hungarian Academy of Sciences and Szent Istvan University, Godollo, Hungary
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Li YH,Cai KJ,Li J,et al. Comparative studies with six extenders for sperm cryopreservation in the cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta)[J]. AMERICAN JOURNAL OF PRIMATOLOGY,2006,68(1):39-49.
APA Li YH,Cai KJ,Li J,Dinnyes A,Ji WZ*,&wji@mail.kiz.ac.cn.(2006).Comparative studies with six extenders for sperm cryopreservation in the cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta).AMERICAN JOURNAL OF PRIMATOLOGY,68(1),39-49.
MLA Li YH,et al."Comparative studies with six extenders for sperm cryopreservation in the cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta)".AMERICAN JOURNAL OF PRIMATOLOGY 68.1(2006):39-49.
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