Effect of sugar type on the survival of frozen-thawed rhesus monkey (Macaca mulatta) sperm

KIZ OpenIR > 生殖与发育生物学

	Effect of sugar type on the survival of frozen-thawed rhesus monkey (Macaca mulatta) sperm
	Si W 1,2; Wang H 1,2; Reid C 3; Hildebrandt TB 3; Ji WZ*1,2; wji@mail.kiz.ac.cn
	2006
发表期刊	AMERICAN JOURNAL OF PRIMATOLOGY
ISSN	0275-2565
卷号	68 期号:1 页码:103-108
合作性质	其它
摘要	Sperm-freezing extenders supplemented with sugar or a combination of different sugars are widely used for the cryopreservation of nonhuman primate spermatozoa. Understanding which sugar or combination of sugars offers the highest level of cryoprotection would be beneficial for the development of sperm-freezing extenders. In the present study we aimed to investigate the effect of glucose, lactose, and raffinose separately or in combination on the cryosurvival of rhesus monkey spermatozoa. Toward that end, we prepared eight extenders by adding various types of sugars to a basic medium (BM): G-BM (0.3 M glucose), L-BM (0.3 M lactose), R-BM (0.3 M raffinose), LG-BM (0.15 M lactose + 0.15 M glucose), RG-BM (0.15 M raffinose + 0.15 M glucose), LR-BM (0.15 M lactose + 0.15 M raffinose), and LRG-BM (0.1 M lactose+0.1 M raffinsoe+0.1 M glucose). A saline control (0.157 M sodium chloride) was also used. The results showed no significant difference in post-thaw motility when spermatozoa were frozen with G-BM, L-BM, LG-BM, RG-BM, and LRG-BM, but the post-thaw motility was significantly lower when it was frozen with R-BM, LR-BM, and the saline control. The highest plasma membrane integrity was achieved when spermatozoa were frozen with G-BM, L-BM, LG-BM, RG-BM, and LRG-BM, and the highest acrosome integrity was achieved with G-BM, L-BM, LG-BM, RG-BM, LRG-BM, and the saline control. The results indicate that the various sugars offered different protective effects. For the cryopreservation of rhesus monkey spermatozoa, glucose (monosaccharide) and lactose (disaccharide) were shown to be more suitable than raffinose (trisaccharide) for preserving spermatozoal motility, plasma membrane, and acrosome. Specifically, raffinose was detrimental to sperm acrosome integrity.
关键词	Rhesus Monkey Sperm Cryopreservation Sugar
资助者	Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KXCX1-05; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009-09; Contract grant number: China National Science Foundation; Contract grant number: 30370166. ; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KXCX1-05; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009-09; Contract grant number: China National Science Foundation; Contract grant number: 30370166. ; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KXCX1-05; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009-09; Contract grant number: China National Science Foundation; Contract grant number: 30370166. ; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KXCX1-05; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009-09; Contract grant number: China National Science Foundation; Contract grant number: 30370166.
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收录类别	SCI
语种	英语
资助者	Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KXCX1-05; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009-09; Contract grant number: China National Science Foundation; Contract grant number: 30370166. ; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KXCX1-05; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009-09; Contract grant number: China National Science Foundation; Contract grant number: 30370166. ; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KXCX1-05; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009-09; Contract grant number: China National Science Foundation; Contract grant number: 30370166. ; Contract grant sponsor: Chinese Academy of Sciences; Contract grant number: KXCX1-05; Contract grant sponsor: Ministry of Science and Technology of China; Contract grant number: 2001DEA10009-09; Contract grant number: China National Science Foundation; Contract grant number: 30370166.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5605
专题	生殖与发育生物学中国科学院昆明灵长类研究中心
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Kunming Primate Research Center, the Chinese Academy of Sciences, Yunnan, China 2.Kunming Institute of Zoology, the Chinese Academy of Sciences, Yunnan, China 3.Institute for Zoo Biology and Wildlife Research, Berlin, Germany
推荐引用方式 GB/T 7714	Si W,Wang H,Reid C,et al. Effect of sugar type on the survival of frozen-thawed rhesus monkey (Macaca mulatta) sperm[J]. AMERICAN JOURNAL OF PRIMATOLOGY,2006,68(1):103-108.
APA	Si W,Wang H,Reid C,Hildebrandt TB,Ji WZ*,&wji@mail.kiz.ac.cn.(2006).Effect of sugar type on the survival of frozen-thawed rhesus monkey (Macaca mulatta) sperm.AMERICAN JOURNAL OF PRIMATOLOGY,68(1),103-108.
MLA	Si W,et al."Effect of sugar type on the survival of frozen-thawed rhesus monkey (Macaca mulatta) sperm".AMERICAN JOURNAL OF PRIMATOLOGY 68.1(2006):103-108.

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