Effects of rhFSH regimen and time interval on ovarian responses to repeated stimulation cycles in rhesus monkeys during a physiologic breeding season

KIZ OpenIR > 生殖与发育生物学

	Effects of rhFSH regimen and time interval on ovarian responses to repeated stimulation cycles in rhesus monkeys during a physiologic breeding season
	Yang S 1,6; Shen Y 2,3; Niu Y 1; Hildebrandt TB 4; JewgenoW K 4; Goeritz F 4; He X 1; Zhou Q 5; Ji W*1; wji@mail.kiz.ac.cn
	2008
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	70 期号:1 页码:108-114
摘要	We studied the effects of repeated stimulation by recombinant human FSH (rhFSH) at various time intervals during a physiologic breeding season in rhesus monkeys. Ovarian recovery and responses were assessed by ultrasonography, serum steroid concentrations, number of oocytes retrieved, and in vitro blastocyst development following IVF. One group underwent a single stimulation regimen with 18 IU rhFSH i.m., followed by 1000 IU hCG, and serum steroid concentrations and ovarian status were determined in the following three menses. Another group was stimulated as before and then allocated into three subgroups; each subgroup was re-stimulated once at the beginning of the ensuing first, second, or third menses. In the final experiment, one group was stimulated with 37.5 IU rhFSH, whereas another group received 18 IU rhFSH. In subsequent cycles, all were re-stimulated twice with 18 IU rhFSH at time intervals of two menstrual cycles (MCs). At the first menses after stimulation, serum progesterone concentrations were significantly higher and the ovaries larger than before stimulation. Monkeys that were re-stimulated at the first menses responded poorly; at the second menses, progesterone concentrations and ovarian size recovered, but the number of oocytes retrieved from re-stimulated monkeys was still significantly reduced. However, animals that were re-stimulated in two MCs later responded well (i.e., percentage of the animals responding, oocytes recovered, and potential for fertilization and blastocyst formation). In conclusion, rhesus monkeys were likely to have similar ovarian responses to repeated stimulation with the same regimen spaced at least two MCs apart
关键词	Rhesus Monkey Ovarian Recovery Repeated Ovarian Stimulation Rhfsh Ultrasonography
资助者	This study was supported by the Chinese Academy of Sciences, KSCX2-YW-R-47, KSCX2-YW-R-089 and Joint Scholar Plan for the Development of Western China; Major State Basic Development Program, 2006CB701505 and 20060102A1070. The authors also thank the German Max-Planck Society for its financial support of the bilateral researchers exchange program. ; This study was supported by the Chinese Academy of Sciences, KSCX2-YW-R-47, KSCX2-YW-R-089 and Joint Scholar Plan for the Development of Western China; Major State Basic Development Program, 2006CB701505 and 20060102A1070. The authors also thank the German Max-Planck Society for its financial support of the bilateral researchers exchange program. ; This study was supported by the Chinese Academy of Sciences, KSCX2-YW-R-47, KSCX2-YW-R-089 and Joint Scholar Plan for the Development of Western China; Major State Basic Development Program, 2006CB701505 and 20060102A1070. The authors also thank the German Max-Planck Society for its financial support of the bilateral researchers exchange program. ; This study was supported by the Chinese Academy of Sciences, KSCX2-YW-R-47, KSCX2-YW-R-089 and Joint Scholar Plan for the Development of Western China; Major State Basic Development Program, 2006CB701505 and 20060102A1070. The authors also thank the German Max-Planck Society for its financial support of the bilateral researchers exchange program.
URL	查看原文
收录类别	SCI
语种	英语
资助者	This study was supported by the Chinese Academy of Sciences, KSCX2-YW-R-47, KSCX2-YW-R-089 and Joint Scholar Plan for the Development of Western China; Major State Basic Development Program, 2006CB701505 and 20060102A1070. The authors also thank the German Max-Planck Society for its financial support of the bilateral researchers exchange program. ; This study was supported by the Chinese Academy of Sciences, KSCX2-YW-R-47, KSCX2-YW-R-089 and Joint Scholar Plan for the Development of Western China; Major State Basic Development Program, 2006CB701505 and 20060102A1070. The authors also thank the German Max-Planck Society for its financial support of the bilateral researchers exchange program. ; This study was supported by the Chinese Academy of Sciences, KSCX2-YW-R-47, KSCX2-YW-R-089 and Joint Scholar Plan for the Development of Western China; Major State Basic Development Program, 2006CB701505 and 20060102A1070. The authors also thank the German Max-Planck Society for its financial support of the bilateral researchers exchange program. ; This study was supported by the Chinese Academy of Sciences, KSCX2-YW-R-47, KSCX2-YW-R-089 and Joint Scholar Plan for the Development of Western China; Major State Basic Development Program, 2006CB701505 and 20060102A1070. The authors also thank the German Max-Planck Society for its financial support of the bilateral researchers exchange program.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5627
专题	生殖与发育生物学科研部门_哺乳动物胚胎发育（郑萍）中国科学院昆明灵长类研究中心
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Kunming Primate Research Center and Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming, Yunnan 650223, PR China 2.National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, PR China 3.Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China 4.Leibniz Institute for Zoo and Wildlife Research, PF 601103, 10252 Berlin, Germany 5.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China 6.Life Science and Technology Research Center, Kunming University of Science and Technology, Kunming 650224, PR China
推荐引用方式 GB/T 7714	Yang S,Shen Y,Niu Y,et al. Effects of rhFSH regimen and time interval on ovarian responses to repeated stimulation cycles in rhesus monkeys during a physiologic breeding season[J]. THERIOGENOLOGY,2008,70(1):108-114.
APA	Yang S.,Shen Y.,Niu Y.,Hildebrandt TB.,JewgenoW K.,...&wji@mail.kiz.ac.cn.(2008).Effects of rhFSH regimen and time interval on ovarian responses to repeated stimulation cycles in rhesus monkeys during a physiologic breeding season.THERIOGENOLOGY,70(1),108-114.
MLA	Yang S,et al."Effects of rhFSH regimen and time interval on ovarian responses to repeated stimulation cycles in rhesus monkeys during a physiologic breeding season".THERIOGENOLOGY 70.1(2008):108-114.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
200870108.pdf（448KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文