Impairments in embryonic genome activation in rhesus monkey somatic cell nuclear transfer embryos

KIZ OpenIR > 生殖与发育生物学

	Impairments in embryonic genome activation in rhesus monkey somatic cell nuclear transfer embryos
	Niu YY 1,2,4; Yang SH 1,4; Yu Y 3; Ding CH 1,4; Yang JF 1,2,4; Wang SF 1,2,4; Ji SH 1,4; He XC 1,4; Xie YH 1,4; Tang XH 1,4; Zhou Q3; Ji WZ1,4; qzhou@ioz.ac.cn ; wji@mail.kiz.ac.cn
	2008
发表期刊	CLONING AND STEM CELLS
ISSN	1536-2302
卷号	10 期号:1 页码:25-35
摘要	Somatic cell nuclear transfer (SCNT) is a remarkable process in which a somatic cell nucleus is acted upon by the ooplasm via mechanisms that today remain unknown. Here we show the developmental competence (% blastocyst) of embryos derived from SCNT (21%) was markedly (p < 0.05) impaired compared with those derived from in vitro fertilization (IVF) (42.1%) in rhesus monkey. Also, SCNT embryos were abnormal in their time course of embryonic development. SCNT produced embryos reached the eight-cell stage faster than did IVF produced embryos. We compare the transcription patterns of five nucleolar-related proteins-nucleolin, nucleophosmin, fibrillarin, PAF53, and UBF-in single IVF and SCNT blastocysts by RT-PCR. The SCNT embryos showed abnormal gene transcription. Immunolocalization of fibrillarin was undetectable in 8-cell and 16-cell SCNT embryos, indicating embryonic genomic activation was delayed in monkey embryos produced by SCNT compared to their IVF-derived counterparts. Some of SCNT embryos appeared to relative higher developmental potential and fibrillarin expression by prolonged exposure of incoming nuclei to a cytoplasm. Thus, our data show that SCNT embryos are characterized by abnormal cleavage and the timely onset of embryonic genome transcription, deficits that may explain their reduced pre- and postimplantation developmental capacity.
资助者	This work was supported by grants from Chinese Academy of Sciences, KSCX1-05-02, KSCX2-YW-R-47 and Joint Scholar Plan for the Development of West- ern China; Major State Basic Development Pro- gram, 2005AA219010 and 2006CB701505; China National Science Foundation 30370166; R&D In- frastructure and Facility Development Program of Yunnan Province 2006PT08. ; This work was supported by grants from Chinese Academy of Sciences, KSCX1-05-02, KSCX2-YW-R-47 and Joint Scholar Plan for the Development of West- ern China; Major State Basic Development Pro- gram, 2005AA219010 and 2006CB701505; China National Science Foundation 30370166; R&D In- frastructure and Facility Development Program of Yunnan Province 2006PT08. ; This work was supported by grants from Chinese Academy of Sciences, KSCX1-05-02, KSCX2-YW-R-47 and Joint Scholar Plan for the Development of West- ern China; Major State Basic Development Pro- gram, 2005AA219010 and 2006CB701505; China National Science Foundation 30370166; R&D In- frastructure and Facility Development Program of Yunnan Province 2006PT08. ; This work was supported by grants from Chinese Academy of Sciences, KSCX1-05-02, KSCX2-YW-R-47 and Joint Scholar Plan for the Development of West- ern China; Major State Basic Development Pro- gram, 2005AA219010 and 2006CB701505; China National Science Foundation 30370166; R&D In- frastructure and Facility Development Program of Yunnan Province 2006PT08.
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收录类别	SCI
语种	英语
资助者	This work was supported by grants from Chinese Academy of Sciences, KSCX1-05-02, KSCX2-YW-R-47 and Joint Scholar Plan for the Development of West- ern China; Major State Basic Development Pro- gram, 2005AA219010 and 2006CB701505; China National Science Foundation 30370166; R&D In- frastructure and Facility Development Program of Yunnan Province 2006PT08. ; This work was supported by grants from Chinese Academy of Sciences, KSCX1-05-02, KSCX2-YW-R-47 and Joint Scholar Plan for the Development of West- ern China; Major State Basic Development Pro- gram, 2005AA219010 and 2006CB701505; China National Science Foundation 30370166; R&D In- frastructure and Facility Development Program of Yunnan Province 2006PT08. ; This work was supported by grants from Chinese Academy of Sciences, KSCX1-05-02, KSCX2-YW-R-47 and Joint Scholar Plan for the Development of West- ern China; Major State Basic Development Pro- gram, 2005AA219010 and 2006CB701505; China National Science Foundation 30370166; R&D In- frastructure and Facility Development Program of Yunnan Province 2006PT08. ; This work was supported by grants from Chinese Academy of Sciences, KSCX1-05-02, KSCX2-YW-R-47 and Joint Scholar Plan for the Development of West- ern China; Major State Basic Development Pro- gram, 2005AA219010 and 2006CB701505; China National Science Foundation 30370166; R&D In- frastructure and Facility Development Program of Yunnan Province 2006PT08.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5629
专题	生殖与发育生物学科研部门_哺乳动物胚胎发育（郑萍）中国科学院昆明灵长类研究中心
通讯作者	qzhou@ioz.ac.cn; wji@mail.kiz.ac.cn
作者单位	1.Kunming Primate Research Center & Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, People’s Republic of China 2.Graduate School of Chinese Academy of Sciences, Beijing, People’s Republic of China 3.Institute of Zoology, Chinese Academy of Sciences, Beijing, People’s Republic of China 4.Yunnan Key Laboratory of Animal Reproductive Biology, Kunming Yunnan, 650223, People’s Republic of China
推荐引用方式 GB/T 7714	Niu YY,Yang SH,Yu Y,et al. Impairments in embryonic genome activation in rhesus monkey somatic cell nuclear transfer embryos[J]. CLONING AND STEM CELLS,2008,10(1):25-35.
APA	Niu YY.,Yang SH.,Yu Y.,Ding CH.,Yang JF.,...&wji@mail.kiz.ac.cn.(2008).Impairments in embryonic genome activation in rhesus monkey somatic cell nuclear transfer embryos.CLONING AND STEM CELLS,10(1),25-35.
MLA	Niu YY,et al."Impairments in embryonic genome activation in rhesus monkey somatic cell nuclear transfer embryos".CLONING AND STEM CELLS 10.1(2008):25-35.

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