| Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus | |
| Zhao LX1,2; Zhu YS[*]1,3; Wang DM4; Chen M1; Gao P1; Xiao WM5; Rao GH3; Wang XH1; Jin HJ1; Xu L6; Sui N*4; Chen Q*1,3; zhuys@nankai.edu.cn, suin@psych.ac.cn and chenq@ioz.ac.cn | |
| 2010 | |
| 发表期刊 | AUTOPHAGY
 |
| ISSN | 1554-8627 |
| 卷号 | 6期号:3页码:386-394 |
| 合作性质 | 其它 |
| 摘要 | Chronic exposure to morphine can induce drug addiction and neural injury, but the exact mechanism is not fully understood. Here we show that morphine induces autophagy in neuroblastoma SH-SY5Y cells and in the rat hippocampus. Pharmacological approach shows that this effect appears to be mediated by PTX-sensitive G protein-coupled receptors signaling cascade. Morphine increases Beclin 1 expression and reduces the interaction between Beclin 1 and Bcl-2, thus releasing Beclin 1 for its pro-autophagic activity. Bcl-2 overexpression inhibits morphine-induced autophagy, whereas knockdown of Beclin 1 or knockout of ATG5 prevents morphine-induced autophagy. In addition, chronic treatment with morphine induces cell death, which is increased by autophagy inhibition through Beclin 1 RNAi. Our data are the first to reveal that Beclin 1 and ATG5 play key roles in morphine-induced autophagy, which may contribute to morphine-induced neuronal injury. |
| 关键词 | Morphine Autophagy Cell Death Beclin 1 Atg5 |
| 资助者 | This work is supported by a key project from the Natural Science Foundation China (No.30630038) and 973 program project (2006CB910102) awarded to Q. Chen, grants from Tianjin Natural Science Foundation (09JCZDJC21200) to Y. Zhu and from National Basic Research Program (2009CB522002) and the NNSF (30770719) to N. Sui. ; This work is supported by a key project from the Natural Science Foundation China (No.30630038) and 973 program project (2006CB910102) awarded to Q. Chen, grants from Tianjin Natural Science Foundation (09JCZDJC21200) to Y. Zhu and from National Basic Research Program (2009CB522002) and the NNSF (30770719) to N. Sui. ; This work is supported by a key project from the Natural Science Foundation China (No.30630038) and 973 program project (2006CB910102) awarded to Q. Chen, grants from Tianjin Natural Science Foundation (09JCZDJC21200) to Y. Zhu and from National Basic Research Program (2009CB522002) and the NNSF (30770719) to N. Sui. ; This work is supported by a key project from the Natural Science Foundation China (No.30630038) and 973 program project (2006CB910102) awarded to Q. Chen, grants from Tianjin Natural Science Foundation (09JCZDJC21200) to Y. Zhu and from National Basic Research Program (2009CB522002) and the NNSF (30770719) to N. Sui. |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | This work is supported by a key project from the Natural Science Foundation China (No.30630038) and 973 program project (2006CB910102) awarded to Q. Chen, grants from Tianjin Natural Science Foundation (09JCZDJC21200) to Y. Zhu and from National Basic Research Program (2009CB522002) and the NNSF (30770719) to N. Sui. ; This work is supported by a key project from the Natural Science Foundation China (No.30630038) and 973 program project (2006CB910102) awarded to Q. Chen, grants from Tianjin Natural Science Foundation (09JCZDJC21200) to Y. Zhu and from National Basic Research Program (2009CB522002) and the NNSF (30770719) to N. Sui. ; This work is supported by a key project from the Natural Science Foundation China (No.30630038) and 973 program project (2006CB910102) awarded to Q. Chen, grants from Tianjin Natural Science Foundation (09JCZDJC21200) to Y. Zhu and from National Basic Research Program (2009CB522002) and the NNSF (30770719) to N. Sui. ; This work is supported by a key project from the Natural Science Foundation China (No.30630038) and 973 program project (2006CB910102) awarded to Q. Chen, grants from Tianjin Natural Science Foundation (09JCZDJC21200) to Y. Zhu and from National Basic Research Program (2009CB522002) and the NNSF (30770719) to N. Sui. |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/5941 |
| 专题 | 科研部门_学习记忆的分子神经机制（徐林） 科研部门_动物模型与人类重大疾病机理重点实验室 |
| 通讯作者 | zhuys@nankai.edu.cn, suin@psych.ac.cn and chenq@ioz.ac.cn |
| 作者单位 | 1.Laboratory of Apoptosis and cancer Biology; The state Key Laboratory of Biomembrane and Membrane Biotechnology; institute of Zoology; chinese Academy of sciences; P.R. china 2.Graduate University of the chinese Academy of sciences; P.R. china 3.college of Life sciences; Nankai University; P.R. china 4.Key Laboratory of Mental health; institute of Psychology; chinese Academy of sciences; P.R. china 5.college of Life sciences; Peking University; P.R. china 6.Key Laboratory of Animal Models and human Disease Mechanisms; Kunming institute of Zoology; chinese Academy of sciences; Kunming, P.R. china |
| 推荐引用方式 GB/T 7714 | Zhao LX,Zhu YS[*],Wang DM,et al. Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus[J]. AUTOPHAGY,2010,6(3):386-394. |
| APA | Zhao LX.,Zhu YS[*].,Wang DM.,Chen M.,Gao P.,...&zhuys@nankai.edu.cn, suin@psych.ac.cn and chenq@ioz.ac.cn.(2010).Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus.AUTOPHAGY,6(3),386-394. |
| MLA | Zhao LX,et al."Morphine induces Beclin 1-and ATG5-dependent autophagy in human neuroblastoma SH-SY5Y cells and in the rat hippocampus".AUTOPHAGY 6.3(2010):386-394. |
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| 201063386.pdf（7152KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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