The nuclear matrix of Euglena gracilis (Euglenophyta): A stage of nuclear matrix evolution?

	The nuclear matrix of Euglena gracilis (Euglenophyta): A stage of nuclear matrix evolution?
	Wen JF*; wenjf@mail.kiz.ac.cn
	2000
发表期刊	BIOLOGY OF THE CELL
ISSN	0248-4900
卷号	92 期号:2 页码:125-131
摘要	Euglena gracilis cell was extracted sequentially with CSK-Triton buffer, RSB-Magik solution and DNase-As solution. DGD embedment-free electron microscopy showed that in the extracted nucleus there was a residual non-chromatin fibrous network. That it could not be removed by hot trichloroacetic acid further supported the idea that it was a non-histone, non-chromatin fibrous protein network, and should be the internal network of the nuclear matrix. After the sequential extraction, the nuclear membrane was removed, leaving behind a layer of lamina; the chromatin was digested and eluted from the dense chromosomes and residual chromosomal structures that should be chromosomal scaffold were revealed. Western blot analysis with antiserum against rat lamins showed that nuclear lamina of the cell possessed two positive polypeptides, a major one and a minor one, which had molecular masses similar to lamin B and lamin A, respectively. Comparing these data with those of the most primitive eukaryote Archezoa and of higher eukaryotes, it was suggested that the lower unicellular eukaryote E. gracilis already had the nuclear matrix structure, and its nuclear matrix (especially the lamina) might represent a stage of evolutionary history of the nuclear matrix.
关键词	Euglena Gracilis Evolution Of Nuclear Matrix Evolution Of Lamins Dgd-embedment Free Electron Microscopy Western Blot
资助者	This work was supported by a grant from the National Natural Science Foundation of China, a grant from the Natural Science Foundation of Yunnan Province (99C0018Q) and a grant for the youth of the Chinese Academy of Sciences (STZ-1-06). ; This work was supported by a grant from the National Natural Science Foundation of China, a grant from the Natural Science Foundation of Yunnan Province (99C0018Q) and a grant for the youth of the Chinese Academy of Sciences (STZ-1-06). ; This work was supported by a grant from the National Natural Science Foundation of China, a grant from the Natural Science Foundation of Yunnan Province (99C0018Q) and a grant for the youth of the Chinese Academy of Sciences (STZ-1-06). ; This work was supported by a grant from the National Natural Science Foundation of China, a grant from the Natural Science Foundation of Yunnan Province (99C0018Q) and a grant for the youth of the Chinese Academy of Sciences (STZ-1-06).
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收录类别	SCI
语种	英语
资助者	This work was supported by a grant from the National Natural Science Foundation of China, a grant from the Natural Science Foundation of Yunnan Province (99C0018Q) and a grant for the youth of the Chinese Academy of Sciences (STZ-1-06). ; This work was supported by a grant from the National Natural Science Foundation of China, a grant from the Natural Science Foundation of Yunnan Province (99C0018Q) and a grant for the youth of the Chinese Academy of Sciences (STZ-1-06). ; This work was supported by a grant from the National Natural Science Foundation of China, a grant from the Natural Science Foundation of Yunnan Province (99C0018Q) and a grant for the youth of the Chinese Academy of Sciences (STZ-1-06). ; This work was supported by a grant from the National Natural Science Foundation of China, a grant from the Natural Science Foundation of Yunnan Province (99C0018Q) and a grant for the youth of the Chinese Academy of Sciences (STZ-1-06).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5947
专题	科研部门_真核细胞进化基因组（文建凡）细胞与分子进化开放实验室
通讯作者	wenjf@mail.kiz.ac.cn
作者单位	Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P.R. Chin
推荐引用方式 GB/T 7714	Wen JF*,wenjf@mail.kiz.ac.cn. The nuclear matrix of Euglena gracilis (Euglenophyta): A stage of nuclear matrix evolution?[J]. BIOLOGY OF THE CELL,2000,92(2):125-131.
APA	Wen JF*,&wenjf@mail.kiz.ac.cn.(2000).The nuclear matrix of Euglena gracilis (Euglenophyta): A stage of nuclear matrix evolution?.BIOLOGY OF THE CELL,92(2),125-131.
MLA	Wen JF*,et al."The nuclear matrix of Euglena gracilis (Euglenophyta): A stage of nuclear matrix evolution?".BIOLOGY OF THE CELL 92.2(2000):125-131.

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