Identification of a Giardia krr1 homolog gene and the secondarily anucleolate condition of Giaridia lamblia

	Identification of a Giardia krr1 homolog gene and the secondarily anucleolate condition of Giaridia lamblia
	Xin DD 1,2; Wen JF*1; He D 1,2; Lu SQ 3; wenjf@mail.kiz.ac.cn
	2005
发表期刊	MOLECULAR BIOLOGY AND EVOLUTION
ISSN	0737-4038
卷号	22 期号:3 页码:391-394
摘要	Giaridia lamblia was long considered to be one of the most primitive eukaryotes and to lie close to the transition between prokaryotes and eukaryotes, but several supporting features, such as lack of mitochondrion and Golgi, have been challenged recently. It was also reported previously that G. lamblia lacked nucleolus, which is the site of pre-rRNA processing and ribosomal assembling in the other eukaryotic cells. Here, we report the identification of the yeast homolog gene, krr1, in the anucleolate eukaryote, G. lamblia. The krr1 gene, encoding one of the pre-rRNA processing proteins in yeast, is actively transcribed in G. lamblia. The deduced protein sequence of G. lamblia krr1 is highly similar to yeast KRR1p that contains a single-KH domain. Our database searches indicated that krr1 genes actually present in diverse eukaryotes and also seem to present in Archaea. However, only the eukaryotic homologs, including that of G. lamblia, have the single-KH domain, which contains the conserved motif KR(K)R. Fibrillarin, another important pre-rRNA processing protein has also been identified previously in G. lamblia. Moreover, our database search shows that nearly half of the other nucleolus-localized protein genes of eukaryotic cells also have their homologs in Giardia. Therefore, we suggest that a common mechanism of pre-RNA processing may operate in the anucleolate eukaryote G. lamblia and in the other eukaryotes and that like the case of "lack of mitochondrion," "lack of nucleolus" may not be a primitive feature, but a secondarily evolutionary condition of the parasite.
关键词	Giardia Lamblia Krr1 Gene Nucleolus Rrna-processing Evolution
资助者	This work was supported by grants (30070362, 30170135, 30021004) from the National Natural Science Foundation of China to J.F.W and a grant (KSCX2-SW-101C) from the Chinese Academy of Sci- ences to J.F.W. ; This work was supported by grants (30070362, 30170135, 30021004) from the National Natural Science Foundation of China to J.F.W and a grant (KSCX2-SW-101C) from the Chinese Academy of Sci- ences to J.F.W. ; This work was supported by grants (30070362, 30170135, 30021004) from the National Natural Science Foundation of China to J.F.W and a grant (KSCX2-SW-101C) from the Chinese Academy of Sci- ences to J.F.W. ; This work was supported by grants (30070362, 30170135, 30021004) from the National Natural Science Foundation of China to J.F.W and a grant (KSCX2-SW-101C) from the Chinese Academy of Sci- ences to J.F.W.
URL	查看原文
收录类别	SCI
资助项目	I
资助者	This work was supported by grants (30070362, 30170135, 30021004) from the National Natural Science Foundation of China to J.F.W and a grant (KSCX2-SW-101C) from the Chinese Academy of Sci- ences to J.F.W. ; This work was supported by grants (30070362, 30170135, 30021004) from the National Natural Science Foundation of China to J.F.W and a grant (KSCX2-SW-101C) from the Chinese Academy of Sci- ences to J.F.W. ; This work was supported by grants (30070362, 30170135, 30021004) from the National Natural Science Foundation of China to J.F.W and a grant (KSCX2-SW-101C) from the Chinese Academy of Sci- ences to J.F.W. ; This work was supported by grants (30070362, 30170135, 30021004) from the National Natural Science Foundation of China to J.F.W and a grant (KSCX2-SW-101C) from the Chinese Academy of Sci- ences to J.F.W.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5963
专题	科研部门_真核细胞进化基因组（文建凡）细胞与分子进化重点实验室
通讯作者	wenjf@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.Graduate School of the Chinese Academy of Sciences, Beijing, China 3.Capital University of Medical Sciences, Beijing, China
推荐引用方式 GB/T 7714	Xin DD,Wen JF*,He D,et al. Identification of a Giardia krr1 homolog gene and the secondarily anucleolate condition of Giaridia lamblia[J]. MOLECULAR BIOLOGY AND EVOLUTION,2005,22(3):391-394.
APA	Xin DD,Wen JF*,He D,Lu SQ,&wenjf@mail.kiz.ac.cn.(2005).Identification of a Giardia krr1 homolog gene and the secondarily anucleolate condition of Giaridia lamblia.MOLECULAR BIOLOGY AND EVOLUTION,22(3),391-394.
MLA	Xin DD,et al."Identification of a Giardia krr1 homolog gene and the secondarily anucleolate condition of Giaridia lamblia".MOLECULAR BIOLOGY AND EVOLUTION 22.3(2005):391-394.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
200522391.pdf（339KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文