Triosephosphate isomerase genes in two trophic modes of euglenoids (Euglenophyceae) and their phylogenetic analysis

	Triosephosphate isomerase genes in two trophic modes of euglenoids (Euglenophyceae) and their phylogenetic analysis
	Sun GL 1,2; Shen W 1,2; Wen JF*1; wenjf@mail.kiz.ac.cn
	2008
发表期刊	JOURNAL OF EUKARYOTIC MICROBIOLOGY
ISSN	1066-5234
卷号	55 期号:3 页码:170-177
合作性质	其它
摘要	Two different length cDNAs encoding triosephosphate isomerase (TIM) were identified in the two trophic modes of euglenoids, the phototrophic Euglena gracilis and Euglena intermedia and the saprotrophic Astasia longa. Sequence analyses and presequence prediction indicated that the shorter cDNA encodes a cytosolic TIM and the longer cDNA encodes a plastid TIM (pTIM). The typical presequence of the putative A. longa pTIM and the high sequence similarity between A. longa pTIM and E. gracilis pTIM imply that A. longa pTIM is targeted to plastids. Therefore, although the plastids of A. longa have lost the ability of photosynthesis, they might retain other TIM-related function(s), such as glycolysis and the synthesis of isopentenyl diphosphate or fatty acids. Including the TIM sequences obtained by us from chlorophytes and rhodophytes, our phylogenetic analyses indicated that euglenoid TIMs group neither with TIMs of kinetoplastids, which share the nearest common ancestor with euglenoids, nor are closely related to TIMs of chlorophytes, which are considered to be the donors of euglenoid plastids through secondary endosymbiosis. Instead, they group with TIMs of rhodophytes. In addition, our amino acid sequence alignment and structure modeling showed that TIMs of euglenoids and rhodophytes share a unique 2-aa insertion within their loop-4 areas. Therefore, either tim convergent evolution or lateral gene transfer (more probably) might have occurred between euglenoids and rhodophytes after the divergence of euglenoids with kinetoplastids.
关键词	Astasia Longa Chlorophytes Euglena Gracilis Kinetoplastids Phylogeny Plastids Rhodophytes
资助者	Financial support for this study was provided by the National Nature Science Foundation of China (30570241; 30021004; 90408016; 30623007), 973 Program (2007CB815705) and the CAS (XiBu ZhiGuang Project 2003; The Knowledge Innovation Program KSCX2-YW-R-091). ; Financial support for this study was provided by the National Nature Science Foundation of China (30570241; 30021004; 90408016; 30623007), 973 Program (2007CB815705) and the CAS (XiBu ZhiGuang Project 2003; The Knowledge Innovation Program KSCX2-YW-R-091). ; Financial support for this study was provided by the National Nature Science Foundation of China (30570241; 30021004; 90408016; 30623007), 973 Program (2007CB815705) and the CAS (XiBu ZhiGuang Project 2003; The Knowledge Innovation Program KSCX2-YW-R-091). ; Financial support for this study was provided by the National Nature Science Foundation of China (30570241; 30021004; 90408016; 30623007), 973 Program (2007CB815705) and the CAS (XiBu ZhiGuang Project 2003; The Knowledge Innovation Program KSCX2-YW-R-091).
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收录类别	SCI
语种	英语
资助者	Financial support for this study was provided by the National Nature Science Foundation of China (30570241; 30021004; 90408016; 30623007), 973 Program (2007CB815705) and the CAS (XiBu ZhiGuang Project 2003; The Knowledge Innovation Program KSCX2-YW-R-091). ; Financial support for this study was provided by the National Nature Science Foundation of China (30570241; 30021004; 90408016; 30623007), 973 Program (2007CB815705) and the CAS (XiBu ZhiGuang Project 2003; The Knowledge Innovation Program KSCX2-YW-R-091). ; Financial support for this study was provided by the National Nature Science Foundation of China (30570241; 30021004; 90408016; 30623007), 973 Program (2007CB815705) and the CAS (XiBu ZhiGuang Project 2003; The Knowledge Innovation Program KSCX2-YW-R-091). ; Financial support for this study was provided by the National Nature Science Foundation of China (30570241; 30021004; 90408016; 30623007), 973 Program (2007CB815705) and the CAS (XiBu ZhiGuang Project 2003; The Knowledge Innovation Program KSCX2-YW-R-091).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5967
专题	科研部门_真核细胞进化基因组（文建凡）遗传资源与进化国家重点实验室
通讯作者	wenjf@mail.kiz.ac.cn
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Sun GL,Shen W,Wen JF*,et al. Triosephosphate isomerase genes in two trophic modes of euglenoids (Euglenophyceae) and their phylogenetic analysis[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2008,55(3):170-177.
APA	Sun GL,Shen W,Wen JF*,&wenjf@mail.kiz.ac.cn.(2008).Triosephosphate isomerase genes in two trophic modes of euglenoids (Euglenophyceae) and their phylogenetic analysis.JOURNAL OF EUKARYOTIC MICROBIOLOGY,55(3),170-177.
MLA	Sun GL,et al."Triosephosphate isomerase genes in two trophic modes of euglenoids (Euglenophyceae) and their phylogenetic analysis".JOURNAL OF EUKARYOTIC MICROBIOLOGY 55.3(2008):170-177.

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