Immunolocalization and expression of vascular endothelial growth factor receptors (VEGFRs) and neuropilins (NRPs) on Keratinocytes in human epidermis

KIZ OpenIR > 科研部门 > 疾病机理遗传学和进化医学学科组（姚永刚）

	Immunolocalization and expression of vascular endothelial growth factor receptors (VEGFRs) and neuropilins (NRPs) on Keratinocytes in human epidermis
	Man XY 1; Yang XH 1; Cai SQ 1; Yao YG 2; Zheng M*1; minz@zju.edu.cn
	2006
发表期刊	MOLECULAR MEDICINE
ISSN	1076-1551
卷号	12 期号:37810 页码:127-136
合作性质	其它
摘要	Vascular endothelial growth factor (VEGF) plays an important role in normal and pathological angiogenesis. VEGF receptors (VEGFRs, including VEGFR-1, VEGFR-2, and VEGFR-3) and neuropilins (NRPs, including NRP-1 and NRF-2) are high-affinity receptors for VEGF and are typically considered to be specific for endothelial cells. Here we showed expression of VEGFRs and NRPs on cultured epidermal keratinocytes at both mRNA and protein levels. We further localized these receptors by immunofluorescence (IF) staining in the epidermis of surgical skin specimens. We found positive staining for VEGFRs and NRPs in all layers of the epidermis except for the stratum corneum. VEGFR-1 and VEGFR-2 are primarily expressed on the cytoplasmic membrane of basal cells and the adjacent spinosum keratinocytes. All layers of the epidermis except for the horny cell layer demonstrated a uniform pattern of VEGFR-3, NRP-1, and NRP-2. Sections staining for NRP-1 and NRP-2 also showed diffuse intense fluorescence and were localized to the cell membrane and cytoplasm of keratinocytes. In another panel of experiments, keratinocytes were treated with different concentrations of VEGF, with or without VEGFR-2 neutralizing antibody in culture. VEGF enhanced the proliferation and migration of keratinocytes, and these effects were partially inhibited by pretreatment with VEGFR-2 neutralizing antibody. Adhesion of keratinocytes to type IV collagen-coated culture plates was decreased by VEGF treatment, but this reduction could be completely reversed by pretreatment with VEGFR-2 neutralizing antibody. Taken together, our results suggest that the expression of VEGFRs and NRPs on keratinocytes may constitute important regulators for its activity and may possibly be responsible for the autocrine signaling in the epidermis.
资助者	This work was supported by a grant (no. 30471565) from the National Natural Science Foundation of China (NSFC). ; This work was supported by a grant (no. 30471565) from the National Natural Science Foundation of China (NSFC). ; This work was supported by a grant (no. 30471565) from the National Natural Science Foundation of China (NSFC). ; This work was supported by a grant (no. 30471565) from the National Natural Science Foundation of China (NSFC).
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语种	英语
资助项目	Immunolocalization and expression of vascular endothelial growth factor receptors (VEGFRs) and neuropilins (NRPs) on Keratinocytes in human epidermis
资助者	This work was supported by a grant (no. 30471565) from the National Natural Science Foundation of China (NSFC). ; This work was supported by a grant (no. 30471565) from the National Natural Science Foundation of China (NSFC). ; This work was supported by a grant (no. 30471565) from the National Natural Science Foundation of China (NSFC). ; This work was supported by a grant (no. 30471565) from the National Natural Science Foundation of China (NSFC).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/5983
专题	科研部门_疾病机理遗传学和进化医学学科组（姚永刚）
通讯作者	minz@zju.edu.cn
作者单位	1.Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009 P.R. China 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
推荐引用方式 GB/T 7714	Man XY,Yang XH,Cai SQ,et al. Immunolocalization and expression of vascular endothelial growth factor receptors (VEGFRs) and neuropilins (NRPs) on Keratinocytes in human epidermis[J]. MOLECULAR MEDICINE,2006,12(37810):127-136.
APA	Man XY,Yang XH,Cai SQ,Yao YG,Zheng M*,&minz@zju.edu.cn.(2006).Immunolocalization and expression of vascular endothelial growth factor receptors (VEGFRs) and neuropilins (NRPs) on Keratinocytes in human epidermis.MOLECULAR MEDICINE,12(37810),127-136.
MLA	Man XY,et al."Immunolocalization and expression of vascular endothelial growth factor receptors (VEGFRs) and neuropilins (NRPs) on Keratinocytes in human epidermis".MOLECULAR MEDICINE 12.37810(2006):127-136.

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