Embryonic expression and evolutionary analysis of the amphioxus Dickkopf and Kremen family genes

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	Embryonic expression and evolutionary analysis of the amphioxus Dickkopf and Kremen family genes
	Zhang YJ 1,2; Mao BY*1; mao@mail.kiz.ac.cn
	2010
发表期刊	JOURNAL OF GENETICS AND GENOMICS
卷号	37 期号:9 页码:637-645
合作性质	其它
摘要	The secreted Wnt signaling inhibitor Dickkopf1 (Dkk1) plays key role in vertebrate head induction. Its receptor Kremen synergizes with Dkk1 in Wnt inhibition. Here we have carried out expression and functional studies of the Dkk and Kremen genes in amphioxus (Branchiostoma belcheri). During embryonic and larval development, BbDkk1/2/4 is expressed in the posterior mesoendoderm, anterior somatic mesoderm and the pharyngeal regions. Its expression becomes restricted to the pharyngeal region on the left side at larval stages. In 45 h larvae, BbDkk1/2/4 is expressed specifically in the cerebral vesicle. BbDkk3 was only detected at larval stages in the mid-intestine region. Seven Kremen related genes were identified in the genome of the Florida amphioxus (Branchiostoma floridae), clustered in 4 scaffolds, and are designated Kremen1-4 and Kremen-like 1-3, respectively. In B. belcheri, Kremen1 is strongly expressed in the mesoendoderm during early development and Kremen3 is expressed asymmetrically in spots in the larval pharyngeal region. In luciferase reporter assays, BbDkk1/2/4 can strongly inhibit Wnt signaling, while BbDkk3, BbKremen1 and BbKremen3 can not. No co-operative effect was observed between amphioxus Dkk1/2/4 and Kremens, suggesting that the interaction between Dkk and Kremen likely originated later during evolution
关键词	Amphioxus Dickkopf Kremen Evolution Expression Patter
资助者	This work was supported by the grant from the Innova- tion Project of Chinese Academy of Sciences (No. KSCX2-YW-R-090). We thank Prof. Junyuan Chen (Nan- jing University) for amphioxus materials. ; This work was supported by the grant from the Innova- tion Project of Chinese Academy of Sciences (No. KSCX2-YW-R-090). We thank Prof. Junyuan Chen (Nan- jing University) for amphioxus materials. ; This work was supported by the grant from the Innova- tion Project of Chinese Academy of Sciences (No. KSCX2-YW-R-090). We thank Prof. Junyuan Chen (Nan- jing University) for amphioxus materials. ; This work was supported by the grant from the Innova- tion Project of Chinese Academy of Sciences (No. KSCX2-YW-R-090). We thank Prof. Junyuan Chen (Nan- jing University) for amphioxus materials.
收录类别	SCI
语种	英语
资助者	This work was supported by the grant from the Innova- tion Project of Chinese Academy of Sciences (No. KSCX2-YW-R-090). We thank Prof. Junyuan Chen (Nan- jing University) for amphioxus materials. ; This work was supported by the grant from the Innova- tion Project of Chinese Academy of Sciences (No. KSCX2-YW-R-090). We thank Prof. Junyuan Chen (Nan- jing University) for amphioxus materials. ; This work was supported by the grant from the Innova- tion Project of Chinese Academy of Sciences (No. KSCX2-YW-R-090). We thank Prof. Junyuan Chen (Nan- jing University) for amphioxus materials. ; This work was supported by the grant from the Innova- tion Project of Chinese Academy of Sciences (No. KSCX2-YW-R-090). We thank Prof. Junyuan Chen (Nan- jing University) for amphioxus materials.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/6345
专题	科研部门_发育生物学（毛炳宇）遗传资源与进化国家重点实验室
通讯作者	mao@mail.kiz.ac.cn
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Zhang YJ,Mao BY*,mao@mail.kiz.ac.cn. Embryonic expression and evolutionary analysis of the amphioxus Dickkopf and Kremen family genes[J]. JOURNAL OF GENETICS AND GENOMICS,2010,37(9):637-645.
APA	Zhang YJ,Mao BY*,&mao@mail.kiz.ac.cn.(2010).Embryonic expression and evolutionary analysis of the amphioxus Dickkopf and Kremen family genes.JOURNAL OF GENETICS AND GENOMICS,37(9),637-645.
MLA	Zhang YJ,et al."Embryonic expression and evolutionary analysis of the amphioxus Dickkopf and Kremen family genes".JOURNAL OF GENETICS AND GENOMICS 37.9(2010):637-645.

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