Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium

KIZ OpenIR > 生殖与发育生物学

	Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium
	Ji SH 1,3,4; Jin LF1,2; Guo XY 1,3,4; Ji WZ1,3,5; lifangj@sohu.com ; wji@mail.kiz.ac.cn
	2010
发表期刊	IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
卷号	46 期号:8 页码:693-701
合作性质	其它
摘要	Studies with hepatic progenitor cells from non-human primates would allow better understanding of their human counterparts. In this study, rhesus monkey liver epithelial progenitor cells (mLEPCs) were derived from a small piece of newborn livers in chemical defined serum-free medium. Digested hepatic cells were treated in Ca2+-containing medium to form cell aggregates. Two types of cell aggregates were generated: elongated spindle cells and polygonal epithelial cells. Elongated spindle cells were expressed as vimentin and brachyury, and they were disappeared within 5 d in our cultures. The remaining type consisted of small polygonal epithelial cells that expressed cytokeratin 7 (CK7), CK8, CK18, nestin, CD49f, and E-cad, the markers of hepatic stem cells, but were negative for alpha-fetoprotein, albumin, and CK19. They can proliferate and be passaged, if on laminin or rat tail collagen gel, to initiate colonies. When cultured with dexamethasone and oncostatin M, the expression of mature hepatocyte markers, such as alpha-1-antitrypsin, intracytoplasmic glycogen storage, indocyanine green uptake, and lipid droplet generation, were induced in differentiated cells. If transferred onto mouse embryonic fibroblasts feeders, they gave rise to CK19-positive cholangiocytes with formation of doughnut-like structure. Thus, mLEPCs with bipotency were derived from newborn monkey liver and may serve as a preclinical model for assessment of cell therapy in humans
关键词	Hepatic Progenitor Cells Bipotency Serum Free Extracelullar Matrix Rhesus Monkey
资助者	This work was supported by research grants from Zhejiang Open Foundation of the Most Important Subjects SWYX0907, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Development Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501- 028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425. ; This work was supported by research grants from Zhejiang Open Foundation of the Most Important Subjects SWYX0907, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Development Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501- 028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425. ; This work was supported by research grants from Zhejiang Open Foundation of the Most Important Subjects SWYX0907, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Development Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501- 028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425. ; This work was supported by research grants from Zhejiang Open Foundation of the Most Important Subjects SWYX0907, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Development Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501- 028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425.
收录类别	SCI
语种	英语
资助者	This work was supported by research grants from Zhejiang Open Foundation of the Most Important Subjects SWYX0907, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Development Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501- 028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425. ; This work was supported by research grants from Zhejiang Open Foundation of the Most Important Subjects SWYX0907, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Development Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501- 028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425. ; This work was supported by research grants from Zhejiang Open Foundation of the Most Important Subjects SWYX0907, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Development Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501- 028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425. ; This work was supported by research grants from Zhejiang Open Foundation of the Most Important Subjects SWYX0907, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Development Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501- 028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/6347
专题	生殖与发育生物学中国科学院昆明灵长类研究中心
通讯作者	lifangj@sohu.com; wji@mail.kiz.ac.cn
作者单位	1.Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, Kunming, Yunnan 650223, China 2.College of Life Science, Shaoxing University, 900# Chennan Dadao, Shaoxing, Zhejiang 312000, China 3.Yunnan Key Laboratory of Animal Reproductive Biology, Kunming, Yunnan, China 4.Graduate School, Chinese Academy of Sciences, Beijing, China 5.Kunming Biomed International, Kunming, Yunnan, China
推荐引用方式 GB/T 7714	Ji SH,Jin LF*,Guo XY,et al. Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2010,46(8):693-701.
APA	Ji SH,Jin LF,Guo XY,Ji WZ,lifangj@sohu.com,&wji@mail.kiz.ac.cn.(2010).Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,46(8),693-701.
MLA	Ji SH,et al."Culture of newborn monkey liver epithelial progenitor cells in chemical defined serum-free medium".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 46.8(2010):693-701.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2010110108.PDF（546KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文