Directional freezing as an alternative method for cryopreserving rhesus macaque (Macaca mulatta) sperm

KIZ OpenIR > 生殖与发育生物学

	Directional freezing as an alternative method for cryopreserving rhesus macaque (Macaca mulatta) sperm
	Si W 1; Lu YQ 1; He XC 1; Ji SH 1; Niu YY 1; Tan T 1; Ji WZ*1,2; wji@mail.kiz.ac.cn
	2010
发表期刊	THERIOGENOLOGY
卷号	74 期号:8 页码:1431-1438
合作性质	其它
摘要	The objective was to develop a freezing protocol using a directional freezing (DF) technique for cryopreservation of rhesus macaque sperm and achieve a survival rate comparable to that achieved with a conventional freezing (CF) technique. Rhesus macaque sperm frozen with a DF technique, with cooling rates of 12 or 16 °C/min, had higher post-thaw motility (P < 0.05) than those cooled at 7 °C/min (59.3, 61.1, and 50.3%, respectively). Furthermore, sperm cryopreserved with 5% glycerol and a DF technique had similar frozen-thawed sperm motility to those cryopreserved by a CF technique (63.7 vs. 53.9%, P > 0.05). The function of sperm cryopreserved at the optimized cooling rate using a DF technique was evaluated by in vitro fertilization of oocytes collected from gonadotropin-stimulated rhesus macaques. Of the 38 mature oocytes collected, 78.9% were fertilized and 71.1, 47.4, and 42.1% of the oocytes developed to the 2-cell, morulae, and blastocyst stages, respectively. In conclusion, rhesus macaque sperm was effectively cryopreserved using a DF technique, providing a new and effective method for genetic preservation in this important species.
关键词	Macaque Sperm Cryopreservation Directional Freezing In Vitro Fertilization
资助者	This work was supported by research grant from the National Key Technologies R & D Program of China 2007BAI33B05 and Major State Basic Development Program 2006CB701505 and 2007CB947701. ; This work was supported by research grant from the National Key Technologies R & D Program of China 2007BAI33B05 and Major State Basic Development Program 2006CB701505 and 2007CB947701. ; This work was supported by research grant from the National Key Technologies R & D Program of China 2007BAI33B05 and Major State Basic Development Program 2006CB701505 and 2007CB947701. ; This work was supported by research grant from the National Key Technologies R & D Program of China 2007BAI33B05 and Major State Basic Development Program 2006CB701505 and 2007CB947701.
收录类别	SCI
语种	英语
资助者	This work was supported by research grant from the National Key Technologies R & D Program of China 2007BAI33B05 and Major State Basic Development Program 2006CB701505 and 2007CB947701. ; This work was supported by research grant from the National Key Technologies R & D Program of China 2007BAI33B05 and Major State Basic Development Program 2006CB701505 and 2007CB947701. ; This work was supported by research grant from the National Key Technologies R & D Program of China 2007BAI33B05 and Major State Basic Development Program 2006CB701505 and 2007CB947701. ; This work was supported by research grant from the National Key Technologies R & D Program of China 2007BAI33B05 and Major State Basic Development Program 2006CB701505 and 2007CB947701.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/6348
专题	生殖与发育生物学科研部门_哺乳动物胚胎发育（郑萍）中国科学院昆明灵长类研究中心
通讯作者	wji@mail.kiz.ac.cn
作者单位	1.Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming, Yunnan 650223, People’s Republic of China 2.Kunming Biomed International, Kunming, Yunnan 650500, People’s Republic of China
推荐引用方式 GB/T 7714	Si W,Lu YQ,He XC,et al. Directional freezing as an alternative method for cryopreserving rhesus macaque (Macaca mulatta) sperm[J]. THERIOGENOLOGY,2010,74(8):1431-1438.
APA	Si W.,Lu YQ.,He XC.,Ji SH.,Niu YY.,...&wji@mail.kiz.ac.cn.(2010).Directional freezing as an alternative method for cryopreserving rhesus macaque (Macaca mulatta) sperm.THERIOGENOLOGY,74(8),1431-1438.
MLA	Si W,et al."Directional freezing as an alternative method for cryopreserving rhesus macaque (Macaca mulatta) sperm".THERIOGENOLOGY 74.8(2010):1431-1438.

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