Construction, Characterization, and Chromosomal Mapping of a Fosmid Library of the White-Cheeked Gibbon (Nomascus leucogenys)

KIZ OpenIR > 遗传资源与进化国家重点实验室 > 保护遗传学(细胞库)

	Construction, Characterization, and Chromosomal Mapping of a Fosmid Library of the White-Cheeked Gibbon (Nomascus leucogenys)
	Chen LP 1,2; Ye JP 1,2; Liu Y 1,2; Wang JH 1; Su WT 1; Yang FY1; Nie WH1; y1@sanger.ac.uk ; whnie@mail.kiz.ac.cn
	2007
发表期刊	Genomics, proteomics & bioinformatics
卷号	5 期号:3-4 页码:207-215
合作性质	其它
摘要	Gibbons have experienced extensive karyotype rearrangements during evolution and represent an ideal model for studying the underlying molecular mechanism of evolutionary chromosomal rearrangements. It is anticipated that the cloning and sequence characterization of evolutionary chromosomal breakpoints will provide vital insights into the molecular force that has driven such a radical karyotype reshuffle in gibbons. We constructed and characterized a high-quality fosmid library of the white-cheeked gibbon (Nomascus leucogenys) containing 192,000 non-redundant clones with an average insert size of 38 kb and 2.5-fold genome coverage. By end sequencing of 100 randomly selected fosmid clones, we generated 196 sequence tags for the library. These end-sequenced fosmid clones were then mapped onto the chromosomes of the white-cheeked gibbon by fluorescence in situ hybridization, and no spurious chimeric clone was detected. BLAST search against the human genome showed a good correlation between the number of hit clones and the number of chromosomes, an indication of unbiased chromosomal distribution of the fosmid library. The chromosomal distribution of the mapped clones is also consistent with the BLAST search result against human and white-cheeked gibbon genomes. The fosmid library and the mapped clones will serve as a valuable resource for further studying gibbons' chromosomal rearrangements and the underlying molecular mechanism as well as for comparative genomic study in the lesser apes.
资助者	This work was supported by the National Natural Science Foundation of China (No. 30270719 and 30623007). ; This work was supported by the National Natural Science Foundation of China (No. 30270719 and 30623007). ; This work was supported by the National Natural Science Foundation of China (No. 30270719 and 30623007). ; This work was supported by the National Natural Science Foundation of China (No. 30270719 and 30623007).
收录类别	SCI
语种	英语
资助者	This work was supported by the National Natural Science Foundation of China (No. 30270719 and 30623007). ; This work was supported by the National Natural Science Foundation of China (No. 30270719 and 30623007). ; This work was supported by the National Natural Science Foundation of China (No. 30270719 and 30623007). ; This work was supported by the National Natural Science Foundation of China (No. 30270719 and 30623007).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/6378
专题	遗传资源与进化国家重点实验室_保护遗传学(细胞库) 细胞与分子进化重点实验室
通讯作者	y1@sanger.ac.uk; whnie@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223 2.Graduate School of Chinese Academy of Sciences, Beijing 100039, China
推荐引用方式 GB/T 7714	Chen LP,Ye JP,Liu Y,et al. Construction, Characterization, and Chromosomal Mapping of a Fosmid Library of the White-Cheeked Gibbon (Nomascus leucogenys)[J]. Genomics, proteomics & bioinformatics,2007,5(3-4):207-215.
APA	Chen LP.,Ye JP.,Liu Y.,Wang JH.,Su WT.,...&whnie@mail.kiz.ac.cn.(2007).Construction, Characterization, and Chromosomal Mapping of a Fosmid Library of the White-Cheeked Gibbon (Nomascus leucogenys).Genomics, proteomics & bioinformatics,5(3-4),207-215.
MLA	Chen LP,et al."Construction, Characterization, and Chromosomal Mapping of a Fosmid Library of the White-Cheeked Gibbon (Nomascus leucogenys)".Genomics, proteomics & bioinformatics 5.3-4(2007):207-215.

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