Construction of Replication-deficient Recombinant Adenoviruses Containing HIV-1 Subtype C gp120 and Preliminary Study on Dendritic Cell Vaccine Loaded with gp120
Acquired immunodeficiency syndrome (AIDS) caused by human immunodeficiency virus( HIV) is characterized by serious damage to the immune system. Within this current HIV pandemic, HIV-1 subtype C (HIV-1 C) is the most prevalent subtype. The urgency of designing an effective and safe HIV-1 C vacccine to protect people from HIV/AIDS is absolutely important. It has been shown that the structural env gene, gp120, could be the primary target for HIV-1 vaccine research. In this study, to elicit broadly neutralizing antibodies against HIV-1, HIV-1 C optimized codon usage gp120 was selected as a crucial immunogen. It was constructed into a replication-deficient recombinant adenoviruses vectors Adeasy-1, and its expression in 293 cells was successfully detected by Western Blot. Dendritic cells (DCs), the most potent APCs, have a pivotal role in the initiation and maintenance of immune responses agaist viruses and have been found to process proteins through MHC classⅠcross-presentation pathways for stimulation of CD8+ T cells, and MHC classpathways for activation of CD4Ⅱ+ T cells. Thus, a therapeutic vaccine, in this study, was made of monocyte-derived DCs transfected with gp120 gene. T cell proliferation and production of CD25 and IFN-γ in CD8+ T cells were significantly increased in vitro by DC vaccine stimulation. The further works will focus on fuctions of DC vaccine loaded with gp120 in vivo.
修改评论