Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken

KIZ OpenIR > 科研部门 > 疾病机理遗传学和进化医学学科组（姚永刚）

	Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken
	Zhang ZR 1,3; Zhu Q 1; Yao YG 2; Jiang XS 3; Du HR 4; Liu YP*1; liuyp578@yahoo.com
	2012
发表期刊	MOLECULAR BIOLOGY REPORTS
卷号	39 期号:4 页码:3517-3521
合作性质	其它
摘要	Calpain-3 is a skeletal muscle-specific protease and participates in the regulation of myogenesis. In this study, we quantified the expression of calpain-3 (CAPN3) mRNA in a Chinese local chicken breed (Sichuan Mountainous Black-boned chicken [MB]), to discern the tissue and ontogenic expression pattern. Meanwhile, we compared the CAPN3 mRNA expression pattern in MB chicken at 10 weeks with a commercial meat type chicken line (S01) of the same age to identify the unique expression pattern under different genetic background. A real time quantitative PCR (qRT-PCR) assay was developed for an accurate measurement of its expression in various tissues from chickens at different ages (0, 2, 4, 6, 8, 10, and 12 weeks). Expression of the CAPN3 mRNA was detected in the selected tissues, regardless of age. The breast muscle and leg muscle tissues had a significantly higher expression than the other tissues from the same individual (P < 0.01). Overall, the CAPN3 mRNA level exhibited a "rise-decline" developmental change in detected tissues except for brain. The S01 chicken had a higher expression of the CAPN3 mRNA in detected tissues than the MB chicken at 10 weeks. The present expression data of chicken CAPN3 gene may provide some information to shed light on the tissue and ontogenic expression pattern during chicken development.
关键词	Chicken Capn3 Qrt-pcr Ontogenic Expression Tissue Distribution
资助者	This study was supported by the Program for New Century Excellent Talents in University (NCET-10- 0889), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), the foundation program of Sichuan Animal Science Academy (SASA2010A02), the Ministry of Agriculture of China (No. 2009ZX08009-159B) and Yunnan Province (2009CI119). ; This study was supported by the Program for New Century Excellent Talents in University (NCET-10- 0889), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), the foundation program of Sichuan Animal Science Academy (SASA2010A02), the Ministry of Agriculture of China (No. 2009ZX08009-159B) and Yunnan Province (2009CI119). ; This study was supported by the Program for New Century Excellent Talents in University (NCET-10- 0889), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), the foundation program of Sichuan Animal Science Academy (SASA2010A02), the Ministry of Agriculture of China (No. 2009ZX08009-159B) and Yunnan Province (2009CI119). ; This study was supported by the Program for New Century Excellent Talents in University (NCET-10- 0889), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), the foundation program of Sichuan Animal Science Academy (SASA2010A02), the Ministry of Agriculture of China (No. 2009ZX08009-159B) and Yunnan Province (2009CI119).
收录类别	SCI
语种	英语
资助者	This study was supported by the Program for New Century Excellent Talents in University (NCET-10- 0889), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), the foundation program of Sichuan Animal Science Academy (SASA2010A02), the Ministry of Agriculture of China (No. 2009ZX08009-159B) and Yunnan Province (2009CI119). ; This study was supported by the Program for New Century Excellent Talents in University (NCET-10- 0889), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), the foundation program of Sichuan Animal Science Academy (SASA2010A02), the Ministry of Agriculture of China (No. 2009ZX08009-159B) and Yunnan Province (2009CI119). ; This study was supported by the Program for New Century Excellent Talents in University (NCET-10- 0889), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), the foundation program of Sichuan Animal Science Academy (SASA2010A02), the Ministry of Agriculture of China (No. 2009ZX08009-159B) and Yunnan Province (2009CI119). ; This study was supported by the Program for New Century Excellent Talents in University (NCET-10- 0889), the Science Fund for Young Scholars in Sichuan Province (ZQ 026-017), the foundation program of Sichuan Animal Science Academy (SASA2010A02), the Ministry of Agriculture of China (No. 2009ZX08009-159B) and Yunnan Province (2009CI119).
WOS记录号	WOS:000301108500017
引用统计
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/6900
专题	科研部门_疾病机理遗传学和进化医学学科组（姚永刚）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	liuyp578@yahoo.com
作者单位	1.College of Animal Science and Technology, Sichuan Agricultural University, 46# Xinkang Road, Ya’an, Sichuan 625014, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China 3.Sichuan Animal Science Academy, Chengdu, Sichuan 610066, China 4.Sichuan Dahen Poultry Breeding Company, Chengdu, Sichuan 610066, China
推荐引用方式 GB/T 7714	Zhang ZR,Zhu Q,Yao YG,et al. Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken[J]. MOLECULAR BIOLOGY REPORTS,2012,39(4):3517-3521.
APA	Zhang ZR.,Zhu Q.,Yao YG.,Jiang XS.,Du HR.,...&liuyp578@yahoo.com.(2012).Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken.MOLECULAR BIOLOGY REPORTS,39(4),3517-3521.
MLA	Zhang ZR,et al."Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken".MOLECULAR BIOLOGY REPORTS 39.4(2012):3517-3521.

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