Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis

	Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis
	Chen ZM 1,2; Yang XW 1,3; Liu ZC 1,3; Zeng L 1,3; Lee WH 1; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2012
发表期刊	BIOCHIMIE
卷号	94 期号:2 页码:328-334
合作性质	其它
摘要	The characterization of new natural antimicrobial peptides (AMPs) can help to solve the serious problem of bacterial resistance to currently used antibiotics. In the current study, we analyzed two families of AMPs from the Chinese torrent frog Amolops jingdongensis with a range of bioactivities. The first family of peptides, named jindongenin-1a, is 24 amino acids in length; a BLAST search of jindongenin-1a revealed no sequence similarity with other AMPs. The second family consists of two peptides containing 29 amino acid residues each. These peptides have high sequence similarity with the AMPs of palustrin-2 and are therefore designated palustrin-2AJ1 and palustrin-2AJ2. The cDNA sequences encoding these AMPs have been cloned and the deduced protein sequence of each AMP has been determined by protein sequencing. Sequence and structural analysis showed that each precursor is composed of a putative signal peptide, an N-terminal spacer, a processing site and a disulfide-bridged heptapeptide segment at the C-terminus. We synthesized jindongenin-1a and palustrin-AJ1 to test their antimicrobial, hemolytic, antioxidative and cytotoxic activities. These two peptides showed broad-spectrum antimicrobial activity to standard and clinically isolated strains of bacteria. In addition, they exhibited weak hemolytic activity to human and rabbit erythrocytes under our experimental conditions. Moreover, these peptides also displayed cytotoxic activity against the K562 and HT29 mammalian cell lines and low anti-oxidant activity. These findings provide helpful insight that will be useful in the design of anti-infective peptide agents.
关键词	Antimicrobial Peptide Jindongenin-1a Palustrin-2aj1/2 Multi-activities Amolops Jingdongensis
资助者	This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), the Chinese NationalNatural Science Foundation (30630014, 30870304 and 31071926). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), the Chinese NationalNatural Science Foundation (30630014, 30870304 and 31071926). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), the Chinese NationalNatural Science Foundation (30630014, 30870304 and 31071926). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), the Chinese NationalNatural Science Foundation (30630014, 30870304 and 31071926).
收录类别	SCI
语种	英语
资助者	This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), the Chinese NationalNatural Science Foundation (30630014, 30870304 and 31071926). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), the Chinese NationalNatural Science Foundation (30630014, 30870304 and 31071926). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), the Chinese NationalNatural Science Foundation (30630014, 30870304 and 31071926). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), the Chinese NationalNatural Science Foundation (30630014, 30870304 and 31071926).
WOS记录号	WOS:000300270900007
引用统计
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/6902
专题	科研部门_生物毒素与人类疾病（张云）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, Yunan, China 3.The Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
推荐引用方式 GB/T 7714	Chen ZM,Yang XW,Liu ZC,et al. Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis[J]. BIOCHIMIE,2012,94(2):328-334.
APA	Chen ZM.,Yang XW.,Liu ZC.,Zeng L.,Lee WH.,...&zhangy@mail.kiz.ac.cn.(2012).Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis.BIOCHIMIE,94(2),328-334.
MLA	Chen ZM,et al."Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis".BIOCHIMIE 94.2(2012):328-334.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
2012040503.pdf（793KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文