Establishment, characterization and immortalization of a fibroblast cell line from the Chinese red belly toad Bombina maxima skin

	Establishment, characterization and immortalization of a fibroblast cell line from the Chinese red belly toad Bombina maxima skin
	Xiang Y 1,2; Gao Q 1,3; Su WT 4; Zeng L 1,2; Wang JH 4; Hu Y 4; Nie WH 4; Ma XT 1,2; Zhang Y 1; Lee WH 1; Zhang Y*1; zhangy@mail.kiz.ac.cn
	2012
发表期刊	CYTOTECHNOLOGY
卷号	64 期号:1 页码:95-105
合作性质	其它
摘要	The skin of the amphibian Bombina maxima is rich in biologically active proteins and peptides, most of which have mammalian analogues. The physiological functions of most of the mammalian analogues are still unknown. Thus, Bombina maxima skin may be a promising model to reveal the physiological role of these proteins and peptides because of their large capacity for secretion. To investigate the physiological role of these proteins and peptides in vitro, a fibroblast cell line was successfully established from Bombina maxima tadpole skin. The cell line grew to form a monolayer with cells of a uniform shape and abundant rough endoplasmic reticulum, which are typical characteristics of fibroblasts. Further identification at a molecular level revealed that they strongly expressed the fibroblast marker protein vimentin. The chromosome number of these cells is 2n = 28, and most of them were diploid. Growth property analysis showed that they grew well for 14 passages. However, cells showed decreased proliferative ability after passage 15. Thus, we tried to immortalize the cells through the overexpression of SV40 T antigen. After selecting by G418, cells stably expressed SV40 large T antigen and showed enhanced proliferative ability and increased telomerase activity. Signal transduction analysis revealed functional p42 mitogen-activated protein (MAP) kinase in immortalized Bombina maxima dermal fibroblasts. Primary fibroblast cells and the immortalized fibroblast cells from Bombina maxima cultured in the present study can be used to investigate the physiological role of Bombina maxima skin-secreted proteins and peptides. In addition, the methods for primary cell culturing and cell immortalization will be useful for culturing and immortalizing cells from other types of amphibians
关键词	Bombina Maxima Fibroblast Immortalization Skin Vimentin
资助者	This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), and the Chinese National Natural Science Foundation (30870304). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), and the Chinese National Natural Science Foundation (30870304). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), and the Chinese National Natural Science Foundation (30870304). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), and the Chinese National Natural Science Foundation (30870304).
收录类别	SCI
语种	英语
资助者	This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), and the Chinese National Natural Science Foundation (30870304). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), and the Chinese National Natural Science Foundation (30870304). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), and the Chinese National Natural Science Foundation (30870304). ; This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), and the Chinese National Natural Science Foundation (30870304).
WOS记录号	WOS:000297836400010
引用统计
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/6916
专题	科研部门_生物毒素与人类疾病（张云）科研部门_动物模型与人类重大疾病机理重点实验室遗传资源与进化国家重点实验室遗传资源与进化国家重点实验室_保护遗传学(细胞库)
通讯作者	zhangy@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming 650223, Yunnan, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China 3.Yunnan Academy of Tobacco Science, Kunming 650106, China 4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
推荐引用方式 GB/T 7714	Xiang Y,Gao Q,Su WT,et al. Establishment, characterization and immortalization of a fibroblast cell line from the Chinese red belly toad Bombina maxima skin[J]. CYTOTECHNOLOGY,2012,64(1):95-105.
APA	Xiang Y.,Gao Q.,Su WT.,Zeng L.,Wang JH.,...&zhangy@mail.kiz.ac.cn.(2012).Establishment, characterization and immortalization of a fibroblast cell line from the Chinese red belly toad Bombina maxima skin.CYTOTECHNOLOGY,64(1),95-105.
MLA	Xiang Y,et al."Establishment, characterization and immortalization of a fibroblast cell line from the Chinese red belly toad Bombina maxima skin".CYTOTECHNOLOGY 64.1(2012):95-105.

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