Purification and Characterization of Two New Allergens from the Venom of Vespa magnifica

	Purification and Characterization of Two New Allergens from the Venom of Vespa magnifica
	An S 1,2,5; Chen LL 4; Wei JF 3; Yang XN 1,5; Ma DY 1; Xu XM 4; Xu XQ 1; He SH3; Lu J1; Lai R*1; shaohenghe@hotmail.com ; lujia1979@gmail.com ; rlai@mail.kiz.ac.cn
	2012
发表期刊	PLOS ONE
卷号	7 期号:2 页码:e31920
合作性质	其它
摘要	Due to poor diagnostic facilities and a lack of medical alertness, allergy to Vespa wasps may be underestimated. Few allergens have been identified from Vespa wasps. Possible native allergen proteins were purified from the wasp venoms (WV) (Vespa magnifica Smith) by gel filtration, ion exchange chromatography, respectively. Their sequences were determined by Edman degradation and cDNA cloning. Their allergenicities were assayed by enzyme-linked immunosorbent assay inhibition tests (ELISA-IT), immunoblots, and skin prick tests (SPTs). Their cross allergencities with Tab y 2 and Tab y 5 purified from the horsefly (Tabanus yao Macquart) were also determined. Two native allergens were identified from the WV, respectively. They are a 25-KDa antigen 5 protein (Ag5) (Vesp ma 5) and a 35-KDa hyaluronidase (Vesp ma 2). They represented major allergens in Vespa magnifica by immunoblots and SPTs. ELISA inhibition of pooled sera IgE reactivity to both the WV and the horsefly salivary gland extracts (HSGE) using four purified allergens (Vesp ma 2, Vesp ma 5 and previously purified Tab y 2 and Tab y 5) was significant. Their cross allergenicities were confirmed by ELISA-IT, immunoblots, and SPTs. They represented the cross reactive allergens from wasp and horsefly and proved the so called wasp-horsefly syndrome
资助者	This work was supported by Chinese National Natural Science Foundation (30830021, 30800185, 30972822, 31025025), the Ministry of Science and Technology (2010CB529800, 2009ZX09103-1/091) and the Ministry of Agriculture. ; This work was supported by Chinese National Natural Science Foundation (30830021, 30800185, 30972822, 31025025), the Ministry of Science and Technology (2010CB529800, 2009ZX09103-1/091) and the Ministry of Agriculture. ; This work was supported by Chinese National Natural Science Foundation (30830021, 30800185, 30972822, 31025025), the Ministry of Science and Technology (2010CB529800, 2009ZX09103-1/091) and the Ministry of Agriculture. ; This work was supported by Chinese National Natural Science Foundation (30830021, 30800185, 30972822, 31025025), the Ministry of Science and Technology (2010CB529800, 2009ZX09103-1/091) and the Ministry of Agriculture.
收录类别	SCI
语种	英语
资助者	This work was supported by Chinese National Natural Science Foundation (30830021, 30800185, 30972822, 31025025), the Ministry of Science and Technology (2010CB529800, 2009ZX09103-1/091) and the Ministry of Agriculture. ; This work was supported by Chinese National Natural Science Foundation (30830021, 30800185, 30972822, 31025025), the Ministry of Science and Technology (2010CB529800, 2009ZX09103-1/091) and the Ministry of Agriculture. ; This work was supported by Chinese National Natural Science Foundation (30830021, 30800185, 30972822, 31025025), the Ministry of Science and Technology (2010CB529800, 2009ZX09103-1/091) and the Ministry of Agriculture. ; This work was supported by Chinese National Natural Science Foundation (30830021, 30800185, 30972822, 31025025), the Ministry of Science and Technology (2010CB529800, 2009ZX09103-1/091) and the Ministry of Agriculture.
WOS记录号	WOS:000302918500046
引用统计	被引频次：65[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/6945
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	shaohenghe@hotmail.com; lujia1979@gmail.com; rlai@mail.kiz.ac.cn
作者单位	1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 2.School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China 3.Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China 4.Clinical Laboratory and the Otolaryngological Department, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan, China 5.Graduate School of the Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	An S,Chen LL,Wei JF,et al. Purification and Characterization of Two New Allergens from the Venom of Vespa magnifica[J]. PLOS ONE,2012,7(2):e31920.
APA	An S.,Chen LL.,Wei JF.,Yang XN.,Ma DY.,...&rlai@mail.kiz.ac.cn.(2012).Purification and Characterization of Two New Allergens from the Venom of Vespa magnifica.PLOS ONE,7(2),e31920.
MLA	An S,et al."Purification and Characterization of Two New Allergens from the Venom of Vespa magnifica".PLOS ONE 7.2(2012):e31920.

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