| Potent Anti-HIV Activities and Mechanisms of Action of a Pine Cone Extract from Pinus yunnanensis | |
| Zhang X1,2,3; Yang LM1; Liu GM4; Liu YJ1,3; Zheng CB5; Lv YJ4; Li HZ4; Zheng YT*1; zhengyt@mail.kiz.ac.cn | |
| 2012 | |
| 发表期刊 | MOLECULES
 |
| 卷号 | 17期号:6页码:6916-6929 |
| 摘要 | The anti-HIV activities of a pine cone extract (YNS-PY-F) from Pinus yunnanensis have been evaluated, and its mechanisms of action were also explored. The pine cone extract, YNS-PY-F, potently inhibited HIV-1(IIIB), HIV-1(RF), HIV-1(A17), HIV-1(AO18) and HIV-2(ROD) and induced cytopathic effect in C8166 cells with EC50 values of 0.96 mu g/mL, 1.53 mu g/mL, 0.88 mu g/mL, 7.20 mu g/mL and 6.17 mu g/mL, respectively. The quantification of a p24 production assay showed that YNS-PY-F significantly inhibited the acute replication of HIV-1(IIIB), HIV-1(RF), HIV-1(A17) and HIV-1(AO18) in C8166 cells. An MTT assay showed that YNS-PY-F also significantly inhibited the HIV-1(IIIB) induced cytolysis in MT-4 cells with an EC50 value of 2.22 mu g/mL. The mechanism assays showed that YNS-PY-F had potent inhibitory effects on the fusion between infected cells and uninfected cells, and the activity of HIV-1 reverse transcriptase, with EC50 values of 7.60 mu g/mL and 4.60 mu g/mL, respectively. Overall, these data suggest that the pine cone extract from Pinus yunnanensis has potent inhibitory activities against HIV-1(IIIB), HIV-1(RF), RT inhibitor-resistant strains HIV-1(A17) and HIV-1(AO18), and HIV-2(ROD), and its anti-HIV mechanisms include inhibition of HIV entry and inhibition of reverse transcriptase activity. |
| 关键词 | Pinus Yunnanensis Pine Cone Anti-hiv Activity Reverse Transcriptase Fusion |
| 资助者 | This research was supported in part by grants from the Key Scientific and Technological Program of China (2009ZX09501-029; 2012ZX10001-006; 2012ZX10001-007), the 973 program (2009CB522306), and the National Natural Science Foundation of China (No.30860365; No.81102483). ; This research was supported in part by grants from the Key Scientific and Technological Program of China (2009ZX09501-029; 2012ZX10001-006; 2012ZX10001-007), the 973 program (2009CB522306), and the National Natural Science Foundation of China (No.30860365; No.81102483). ; This research was supported in part by grants from the Key Scientific and Technological Program of China (2009ZX09501-029; 2012ZX10001-006; 2012ZX10001-007), the 973 program (2009CB522306), and the National Natural Science Foundation of China (No.30860365; No.81102483). ; This research was supported in part by grants from the Key Scientific and Technological Program of China (2009ZX09501-029; 2012ZX10001-006; 2012ZX10001-007), the 973 program (2009CB522306), and the National Natural Science Foundation of China (No.30860365; No.81102483). |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | This research was supported in part by grants from the Key Scientific and Technological Program of China (2009ZX09501-029; 2012ZX10001-006; 2012ZX10001-007), the 973 program (2009CB522306), and the National Natural Science Foundation of China (No.30860365; No.81102483). ; This research was supported in part by grants from the Key Scientific and Technological Program of China (2009ZX09501-029; 2012ZX10001-006; 2012ZX10001-007), the 973 program (2009CB522306), and the National Natural Science Foundation of China (No.30860365; No.81102483). ; This research was supported in part by grants from the Key Scientific and Technological Program of China (2009ZX09501-029; 2012ZX10001-006; 2012ZX10001-007), the 973 program (2009CB522306), and the National Natural Science Foundation of China (No.30860365; No.81102483). ; This research was supported in part by grants from the Key Scientific and Technological Program of China (2009ZX09501-029; 2012ZX10001-006; 2012ZX10001-007), the 973 program (2009CB522306), and the National Natural Science Foundation of China (No.30860365; No.81102483). |
| WOS记录号 | WOS:000305800400050 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/7012 |
| 专题 | 科研部门_分子免疫药理学（郑永唐） 科研部门_动物模型与人类重大疾病机理重点实验室 |
| 通讯作者 | zhengyt@mail.kiz.ac.cn |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Science & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.School of Pharmaceutical Science, Kunming Medical University, Kunming 650500, China 3.Graduate School of the Chinese Academy of Science, Beijing 100039, China 4.School of Pharmacy, Dali University, Dali 671000, China; 5.School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China |
| 推荐引用方式 GB/T 7714 | Zhang X,Yang LM,Liu GM,et al. Potent Anti-HIV Activities and Mechanisms of Action of a Pine Cone Extract from Pinus yunnanensis[J]. MOLECULES,2012,17(6):6916-6929. |
| APA | Zhang X.,Yang LM.,Liu GM.,Liu YJ.,Zheng CB.,...&zhengyt@mail.kiz.ac.cn.(2012).Potent Anti-HIV Activities and Mechanisms of Action of a Pine Cone Extract from Pinus yunnanensis.MOLECULES,17(6),6916-6929. |
| MLA | Zhang X,et al."Potent Anti-HIV Activities and Mechanisms of Action of a Pine Cone Extract from Pinus yunnanensis".MOLECULES 17.6(2012):6916-6929. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 2012090424.pdf（366KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
| 个性服务 |
| 推荐该条目 |
| 保存到收藏夹 |
| 查看访问统计 |
| 导出为Endnote文件 |
| 谷歌学术 |
| 谷歌学术中相似的文章 |
| [Zhang X]的文章 |
| [Yang LM]的文章 |
| [Liu GM]的文章 |
| 百度学术 |
| 百度学术中相似的文章 |
| [Zhang X]的文章 |
| [Yang LM]的文章 |
| [Liu GM]的文章 |
| 必应学术 |
| 必应学术中相似的文章 |
| [Zhang X]的文章 |
| [Yang LM]的文章 |
| [Liu GM]的文章 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论