Proteomics and transcriptome analysis coupled with pharmacological test reveals the diversity of anti-thrombosis proteins from the medicinal insect, Eupolyphaga sinensis

	Proteomics and transcriptome analysis coupled with pharmacological test reveals the diversity of anti-thrombosis proteins from the medicinal insect, Eupolyphaga sinensis
	Wang Y 1,4; Yan HL 2; Wang YP 1; Yang HL 1; Wei L 2; Xiao Y 1,4; Ye HH 3; Lai R1,2; Liu R2; rlai@mail.kiz.ac.cn ; liuruisl@163.com
	2012
发表期刊	INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷号	42 期号:8 页码:537-544
合作性质	其它
摘要	The insect of Eupolyphaga sinensis Walker has been used as traditional anti-thrombosis medicine without bleeding risk for several hundreds years in eastern countries. Our previous work has identified a bifunctional anti-thrombosis protein containing both direct-acting fibrin(ogen)olytic and plasminogen-activating activities from the insect. By proteomics and transcriptome analysis, 105 serine proteases belonging to four families were identified from the ground beetle, E. sinensis and the classification is for serine proteases of this organism. Pharmacological test indicated that 5 (eupolytin 1-5) of them have the abilities to hydrolyze fibrin(ogen) and/or activate plasminogen. The current work revealed the extreme diversity of anti-thrombosis components in E. sinensis and anti-thrombosis molecular mechanisms of the traditional medicinal insect, and provided many templates for the development of new thrombolytic agents. Especially, these proteins, which contain both plasmin- and PA (plasminogen-activating)-like activities, are excellent candidates for anti-thrombosis medicines.
关键词	Insect Thrombosis Plasminogen-activator Fibrin(Ogen)Olytic
资助者	This work was supported by Chinese National Natural Science Foundation (30830021, 31000962, 31070701, 31025025), the MinistryofScienceandTechnology(2010CB529800,2009ZX09103- 1/091) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021, 31000962, 31070701, 31025025), the MinistryofScienceandTechnology(2010CB529800,2009ZX09103- 1/091) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021, 31000962, 31070701, 31025025), the MinistryofScienceandTechnology(2010CB529800,2009ZX09103- 1/091) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021, 31000962, 31070701, 31025025), the MinistryofScienceandTechnology(2010CB529800,2009ZX09103- 1/091) and the Ministry of Agriculture (2009ZX08009-159B).
收录类别	SCI
语种	英语
资助者	This work was supported by Chinese National Natural Science Foundation (30830021, 31000962, 31070701, 31025025), the MinistryofScienceandTechnology(2010CB529800,2009ZX09103- 1/091) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021, 31000962, 31070701, 31025025), the MinistryofScienceandTechnology(2010CB529800,2009ZX09103- 1/091) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021, 31000962, 31070701, 31025025), the MinistryofScienceandTechnology(2010CB529800,2009ZX09103- 1/091) and the Ministry of Agriculture (2009ZX08009-159B). ; This work was supported by Chinese National Natural Science Foundation (30830021, 31000962, 31070701, 31025025), the MinistryofScienceandTechnology(2010CB529800,2009ZX09103- 1/091) and the Ministry of Agriculture (2009ZX08009-159B).
WOS记录号	WOS:000306207500002
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/7018
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	rlai@mail.kiz.ac.cn; liuruisl@163.com
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, 1st Weigang, Nanjing, Jiangsu 210095, China 3.The Academy of Military Medical Sciences, Beijing 100009, China 4.Graduate School of the Chinese Academy of Sciences, Beijing, 100009, China
推荐引用方式 GB/T 7714	Wang Y,Yan HL,Wang YP,et al. Proteomics and transcriptome analysis coupled with pharmacological test reveals the diversity of anti-thrombosis proteins from the medicinal insect, Eupolyphaga sinensis[J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,2012,42(8):537-544.
APA	Wang Y.,Yan HL.,Wang YP.,Yang HL.,Wei L.,...&liuruisl@163.com.(2012).Proteomics and transcriptome analysis coupled with pharmacological test reveals the diversity of anti-thrombosis proteins from the medicinal insect, Eupolyphaga sinensis.INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,42(8),537-544.
MLA	Wang Y,et al."Proteomics and transcriptome analysis coupled with pharmacological test reveals the diversity of anti-thrombosis proteins from the medicinal insect, Eupolyphaga sinensis".INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 42.8(2012):537-544.

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