Comparative Analysis of the 5S rRNA and Its Associated Proteins Reveals Unique Primitive Rather Than Parasitic Features in Giardia lamblia

	Comparative Analysis of the 5S rRNA and Its Associated Proteins Reveals Unique Primitive Rather Than Parasitic Features in Giardia lamblia
	Feng JM 1,2; Sun J 1,2; Xin DD 3; Wen JF*1; wenjf@mail.kiz.ac.cn
	2012
发表期刊	PLOS ONE
卷号	7 期号:6 页码:e36878
摘要	Background: 5S rRNA is a highly conserved ribosomal component. Eukaryotic 5S rRNA and its associated proteins (5S rRNA system) have become very well understood. Giardia lamblia was thought by some researchers to be the most primitive extant eukaryote while others considered it a highly evolved parasite. Previous reports have indicated that some aspects of its 5S rRNA system are simpler than that of common eukaryotes. We here explore whether this is true to its entire system, and whether this simplicity is a primitive or parasitic feature. Methodology/Principal Findings: By collecting and confirming pre-existing data and identifying new data, we obtained almost complete datasets of the system of three isolates of G. lamblia, two other parasitic excavates (Trichomonas vaginalis, Trypanosoma cruzi), and one free-living one (Naegleria gruberi). After comprehensively comparing each aspect of the system among these excavates and also with those of archaea and common eukaryotes, we found all the three Giardia isolates to harbor a same simplified 5S rRNA system, which is not only much simpler than that of common eukaryotes but also the simplest one among those of these excavates, and is surprisingly very similar to that of archaea; we also found among these excavates the system in parasitic species is not necessarily simpler than that in free-living species, conversely, the system of free-living species is even simpler in some respects than those of parasitic ones. Conclusion/Significance: The simplicity of Giardia 5S rRNA system should be considered a primitive rather than parasitically-degenerated feature. Therefore, Giardia 5S rRNA system might be a primitive system that is intermediate between that of archaea and the common eukaryotic model system, and it may reflect the evolutionary history of the eukaryotic 5S rRNA system from the archaeal form. Our results also imply G. lamblia might be a primitive eukaryote with secondary parasitically-degenerated features.
资助者	This work was supported by grants from the National Natural Science Foundation of China (30830018; 31172081)and the Knowledge Innovation Program (KSCX2-EW-J-23). ; This work was supported by grants from the National Natural Science Foundation of China (30830018; 31172081)and the Knowledge Innovation Program (KSCX2-EW-J-23). ; This work was supported by grants from the National Natural Science Foundation of China (30830018; 31172081)and the Knowledge Innovation Program (KSCX2-EW-J-23). ; This work was supported by grants from the National Natural Science Foundation of China (30830018; 31172081)and the Knowledge Innovation Program (KSCX2-EW-J-23).
收录类别	SCI
语种	英语
资助者	This work was supported by grants from the National Natural Science Foundation of China (30830018; 31172081)and the Knowledge Innovation Program (KSCX2-EW-J-23). ; This work was supported by grants from the National Natural Science Foundation of China (30830018; 31172081)and the Knowledge Innovation Program (KSCX2-EW-J-23). ; This work was supported by grants from the National Natural Science Foundation of China (30830018; 31172081)and the Knowledge Innovation Program (KSCX2-EW-J-23). ; This work was supported by grants from the National Natural Science Foundation of China (30830018; 31172081)and the Knowledge Innovation Program (KSCX2-EW-J-23).
WOS记录号	WOS:000305351700004
引用统计
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/7041
专题	科研部门_真核细胞进化基因组（文建凡）遗传资源与进化国家重点实验室
通讯作者	wenjf@mail.kiz.ac.cn
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, People’s Republic of China 2.Graduate School of Chinese Academy of Sciences, Beijing, People’s Republic of China 3.College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, People’s Republic of China
推荐引用方式 GB/T 7714	Feng JM,Sun J,Xin DD,et al. Comparative Analysis of the 5S rRNA and Its Associated Proteins Reveals Unique Primitive Rather Than Parasitic Features in Giardia lamblia[J]. PLOS ONE,2012,7(6):e36878.
APA	Feng JM,Sun J,Xin DD,Wen JF*,&wenjf@mail.kiz.ac.cn.(2012).Comparative Analysis of the 5S rRNA and Its Associated Proteins Reveals Unique Primitive Rather Than Parasitic Features in Giardia lamblia.PLOS ONE,7(6),e36878.
MLA	Feng JM,et al."Comparative Analysis of the 5S rRNA and Its Associated Proteins Reveals Unique Primitive Rather Than Parasitic Features in Giardia lamblia".PLOS ONE 7.6(2012):e36878.

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