SPECIES IDENTIFICATION OF TEN COMMON FARM ANIMALS BASED ON MITOCHONDRIAL 12S rRNA GENE POLYMORPHISMS

KIZ OpenIR > 科研部门 > 疾病机理遗传学和进化医学学科组（姚永刚）

	SPECIES IDENTIFICATION OF TEN COMMON FARM ANIMALS BASED ON MITOCHONDRIAL 12S rRNA GENE POLYMORPHISMS
	Chen SY 3; Yao YG 4; Liu YP*3; liuyp578@yahoo.com
	2012
发表期刊	ANIMAL BIOTECHNOLOGY
卷号	23 期号:3 页码:213-220
合作性质	其它
摘要	Bio-techniques such as genetic manipulation, marker-assisted selection, and identity test have largely facilitated the modern animal production practices. In the present study, we established a reliable and cost-effective molecular method of species identification for common farm animals. We first (re-)analyzed 179 mitochondrial 12S rRNA gene sequences of ten farm animal species to determine the intra-species and species-specific variations. The PCR-RFLP method was subsequently designed to identify these species by using endonucleases BshNI, ScaI, AluI, and BfaI. The poultry and livestock species were first discriminated by one double-digestion of both BshNI and ScaI, which generated different fragment patterns (325 bp and 115 bp for poultry vs. 364 bp and 76 bp for livestock). The ten species could be further discerned according to species-specific restriction pattern by subjecting to digestion of AluI and BfaI, respectively. Our approach would be more reliable by taking the intra-species variations into consideration and could be applied to species identity test, commercial fraud, and wildlife crime.
关键词	12s Rrna Gene Farm Animals Mitochondrial Dna Restriction Fragment Length Polymorphism Species Identification
资助者	This work was financially supported by the Ministry of Agriculture of China (2009ZX08009-159B), the Sichuan Province (2011JTD0032, 11TD007), and Yunnan Province (2009CI119). ; This work was financially supported by the Ministry of Agriculture of China (2009ZX08009-159B), the Sichuan Province (2011JTD0032, 11TD007), and Yunnan Province (2009CI119). ; This work was financially supported by the Ministry of Agriculture of China (2009ZX08009-159B), the Sichuan Province (2011JTD0032, 11TD007), and Yunnan Province (2009CI119). ; This work was financially supported by the Ministry of Agriculture of China (2009ZX08009-159B), the Sichuan Province (2011JTD0032, 11TD007), and Yunnan Province (2009CI119).
收录类别	SCI
语种	英语
资助者	This work was financially supported by the Ministry of Agriculture of China (2009ZX08009-159B), the Sichuan Province (2011JTD0032, 11TD007), and Yunnan Province (2009CI119). ; This work was financially supported by the Ministry of Agriculture of China (2009ZX08009-159B), the Sichuan Province (2011JTD0032, 11TD007), and Yunnan Province (2009CI119). ; This work was financially supported by the Ministry of Agriculture of China (2009ZX08009-159B), the Sichuan Province (2011JTD0032, 11TD007), and Yunnan Province (2009CI119). ; This work was financially supported by the Ministry of Agriculture of China (2009ZX08009-159B), the Sichuan Province (2011JTD0032, 11TD007), and Yunnan Province (2009CI119).
WOS记录号	WOS:000307353400007
引用统计
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/7065
专题	科研部门_疾病机理遗传学和进化医学学科组（姚永刚）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	liuyp578@yahoo.com
作者单位	1.Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu Campus, Chengdu, Sichuan, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China 3.Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu Campus, Chengdu, Sichuan, China 4.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan, China
推荐引用方式 GB/T 7714	Chen SY,Yao YG,Liu YP*,et al. SPECIES IDENTIFICATION OF TEN COMMON FARM ANIMALS BASED ON MITOCHONDRIAL 12S rRNA GENE POLYMORPHISMS[J]. ANIMAL BIOTECHNOLOGY,2012,23(3):213-220.
APA	Chen SY,Yao YG,Liu YP*,&liuyp578@yahoo.com.(2012).SPECIES IDENTIFICATION OF TEN COMMON FARM ANIMALS BASED ON MITOCHONDRIAL 12S rRNA GENE POLYMORPHISMS.ANIMAL BIOTECHNOLOGY,23(3),213-220.
MLA	Chen SY,et al."SPECIES IDENTIFICATION OF TEN COMMON FARM ANIMALS BASED ON MITOCHONDRIAL 12S rRNA GENE POLYMORPHISMS".ANIMAL BIOTECHNOLOGY 23.3(2012):213-220.

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