Photoinactivation of cell-free human immunodeficiency virus by hematoporphyrin monomethyl ether

	Photoinactivation of cell-free human immunodeficiency virus by hematoporphyrin monomethyl ether
	Yin HJ 1; Li YX*1; Zheng YT 2; Ye XY 3; Zheng LQ 1; Li CY 2; Xue ZX 1; yingxinli@tijmu.edu.cn
	2012
发表期刊	LASERS IN MEDICAL SCIENCE Volume
卷号	27 期号:5 页码:943-950
摘要	Human immunodeficiency virus (HIV) particles that remain in the blood of patients are frequently ignored as targets for AIDS treatment. We therefore investigated the use of photodynamic therapy (PDT) with hematoporphyrin monomethyl ether (HMME) as a means of inactivating cell-free HIV in vitro. Virus particles including HIV-1(IIIB), resistant HIV-1 variants, HIV-1 clinical variants, and HIV-2 variants were incubated with HMME for 40 min, followed by irradiation with a 630-nm semiconductor laser at an energy density of 0.3 J/cm(2). The antiviral effects were evaluated by counting syncytium formation or measuring p24 antigen expression levels in supernatants by enzyme-linked immunosorbent assay. The relationships between photoinactivation and HMME concentrations, energy density, power density and antioxidants (NaN3 and d-mannitol) were also assessed using the above methods. All the tested virus particles were completely responsive to HMME-PDT. HMME concentration and energy density were positively correlated with photoinactivation of HIV, while power density was negatively correlated. Both sodium azide and d-mannitol weakened the inhibitory effect of PDT on virus-induced membrane fusion, with d-mannitol having a stronger effect. HMME-PDT can inactivate HIV particles, and may therefore represent a promising treatment for AIDS patients.
关键词	Photodynamic Therapy Human Immunodeficiency Virus Photosensitizer Energy Density Power Density Antioxidants
资助者	This work was supported by grants from the Chinese Academy of Medical Sciences (0124, 0217) and the Natural Science Foundation of China (60678047). ; This work was supported by grants from the Chinese Academy of Medical Sciences (0124, 0217) and the Natural Science Foundation of China (60678047). ; This work was supported by grants from the Chinese Academy of Medical Sciences (0124, 0217) and the Natural Science Foundation of China (60678047). ; This work was supported by grants from the Chinese Academy of Medical Sciences (0124, 0217) and the Natural Science Foundation of China (60678047).
收录类别	SCI
语种	英语
资助者	This work was supported by grants from the Chinese Academy of Medical Sciences (0124, 0217) and the Natural Science Foundation of China (60678047). ; This work was supported by grants from the Chinese Academy of Medical Sciences (0124, 0217) and the Natural Science Foundation of China (60678047). ; This work was supported by grants from the Chinese Academy of Medical Sciences (0124, 0217) and the Natural Science Foundation of China (60678047). ; This work was supported by grants from the Chinese Academy of Medical Sciences (0124, 0217) and the Natural Science Foundation of China (60678047).
WOS记录号	WOS:000307406500007
引用统计
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/7067
专题	科研部门_分子免疫药理学（郑永唐）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	yingxinli@tijmu.edu.cn
作者单位	1.Laser Medicine Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Tianjin 300192, China 2.Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 3.Department of Heart Medicine, Tianjin First Center Hospital, Tianjin 300192, China
推荐引用方式 GB/T 7714	Yin HJ,Li YX*,Zheng YT,et al. Photoinactivation of cell-free human immunodeficiency virus by hematoporphyrin monomethyl ether[J]. LASERS IN MEDICAL SCIENCE Volume,2012,27(5):943-950.
APA	Yin HJ.,Li YX*.,Zheng YT.,Ye XY.,Zheng LQ.,...&yingxinli@tijmu.edu.cn.(2012).Photoinactivation of cell-free human immunodeficiency virus by hematoporphyrin monomethyl ether.LASERS IN MEDICAL SCIENCE Volume,27(5),943-950.
MLA	Yin HJ,et al."Photoinactivation of cell-free human immunodeficiency virus by hematoporphyrin monomethyl ether".LASERS IN MEDICAL SCIENCE Volume 27.5(2012):943-950.

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