Intracellular Overexpression of HIV-1 Nef Impairs Differentiation and Maturation of Monocytic Precursors towards Dendritic Cells

KIZ OpenIR > 分子免疫生物学

	Intracellular Overexpression of HIV-1 Nef Impairs Differentiation and Maturation of Monocytic Precursors towards Dendritic Cells
	Guo Y 1; Xu WW 1,2; Song J 1,2; Deng W 1; Liu DQ*1; Zhang HT 1; liudiqiu@mail.kiz.ac.cn
	2012
发表期刊	PLOS ONE
卷号	7 期号:7 页码:e40179
合作性质	其它
摘要	Nef functions as an immunosuppressive factor critical for HIV-1 replication, survival and development of AIDS following HIV-1 infection. What effects Nef exerts on differentiation and maturation of monocytes towards dendritic cells (DCs) remains greatly controversial. In this study, we used THP-1 (human monocytic leukemia cell line) as monocytic DC precursors to investigate how overexpression of HIV-1 Nef influences the processes of differentiation and maturation of dendritic cells. In striking contrast to negative controls, our results showed that morphological and phenotypical changes (CD11c, CD14, CD40, CD80, CD83, CD86, and HLA-DR) occurred on recombinant THP-1 expressing HIV-1 Nef (short for Nef) upon co-stimulation of GM-CSF/IL-4 or GM-CSF/IL-4/TNF-alpha/ionomycin. Moreover, CD4, CCR5, and CXCR4 were also down-regulated on Nef. It might be hypothesized that Nef prevents superinfection and signal transduction in HIV-1 infected monocytes. Collectively, our study demonstrates that long-lasting expression of Nef at high levels indeed retards differentiation and maturation of dendritic cells in terms of phenotype and morphology. We are hopeful that potentially, stable expression of intracellular Nef in vivo may function as a subtle mode to support long-lasting HIV-1 existence.
资助者	This work was supported by the 100 Talents Programme of The Chinese Academy of 13 Sciences(No. A0820), the National Natural Science Foundation of China (General program, No. 31070817 and No. 30771951), and the Applied Fundamental Research Program of Yunnan Province (No.2008CC001 and No. 2010CD101). ; This work was supported by the 100 Talents Programme of The Chinese Academy of 13 Sciences(No. A0820), the National Natural Science Foundation of China (General program, No. 31070817 and No. 30771951), and the Applied Fundamental Research Program of Yunnan Province (No.2008CC001 and No. 2010CD101). ; This work was supported by the 100 Talents Programme of The Chinese Academy of 13 Sciences(No. A0820), the National Natural Science Foundation of China (General program, No. 31070817 and No. 30771951), and the Applied Fundamental Research Program of Yunnan Province (No.2008CC001 and No. 2010CD101). ; This work was supported by the 100 Talents Programme of The Chinese Academy of 13 Sciences(No. A0820), the National Natural Science Foundation of China (General program, No. 31070817 and No. 30771951), and the Applied Fundamental Research Program of Yunnan Province (No.2008CC001 and No. 2010CD101).
收录类别	SCI
语种	英语
资助者	This work was supported by the 100 Talents Programme of The Chinese Academy of 13 Sciences(No. A0820), the National Natural Science Foundation of China (General program, No. 31070817 and No. 30771951), and the Applied Fundamental Research Program of Yunnan Province (No.2008CC001 and No. 2010CD101). ; This work was supported by the 100 Talents Programme of The Chinese Academy of 13 Sciences(No. A0820), the National Natural Science Foundation of China (General program, No. 31070817 and No. 30771951), and the Applied Fundamental Research Program of Yunnan Province (No.2008CC001 and No. 2010CD101). ; This work was supported by the 100 Talents Programme of The Chinese Academy of 13 Sciences(No. A0820), the National Natural Science Foundation of China (General program, No. 31070817 and No. 30771951), and the Applied Fundamental Research Program of Yunnan Province (No.2008CC001 and No. 2010CD101). ; This work was supported by the 100 Talents Programme of The Chinese Academy of 13 Sciences(No. A0820), the National Natural Science Foundation of China (General program, No. 31070817 and No. 30771951), and the Applied Fundamental Research Program of Yunnan Province (No.2008CC001 and No. 2010CD101).
WOS记录号	WOS:000306354700029
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/7069
专题	分子免疫生物学科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	liudiqiu@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, China 2.Graduate School of the Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	Guo Y,Xu WW,Song J,et al. Intracellular Overexpression of HIV-1 Nef Impairs Differentiation and Maturation of Monocytic Precursors towards Dendritic Cells[J]. PLOS ONE,2012,7(7):e40179.
APA	Guo Y.,Xu WW.,Song J.,Deng W.,Liu DQ*.,...&liudiqiu@mail.kiz.ac.cn.(2012).Intracellular Overexpression of HIV-1 Nef Impairs Differentiation and Maturation of Monocytic Precursors towards Dendritic Cells.PLOS ONE,7(7),e40179.
MLA	Guo Y,et al."Intracellular Overexpression of HIV-1 Nef Impairs Differentiation and Maturation of Monocytic Precursors towards Dendritic Cells".PLOS ONE 7.7(2012):e40179.

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