Amphibian skin is a morphologically, biochemically and physiologically complex organ that fulfills a wide range of functions necessary for amphibian survival. A serious functions takes place in the skin, including respiration, water regulation, antimicrobial defense, mechanic damage defense, etc. A number of diverse bioactive components have been isolated and characterized from the amphibian skin secretion and would be contribute to the searching and recognition of physiological function in the amphibian skin.Following the trypsin degeneration, two EP sequences of peroxiredoxin VI were sequencing and provided an evidence of BMS –Prdx VI from the skin secretion .With the identification of this protein by Western Blot，BMS-Prdx VI was purified and characterized form frog Bombina Maxima skin secretion by three steps protocol including DEAE-Sephadex A-50 ion exchange，Sephadex G-100，Sephadex G-50 gel filtration. BMS-Prdx VI is composed of a single peptide chain demonstrated by SDS-PAGE. Under reducing conditions and non-reducing conditions, its apparent molecular weight is 26 kDa. Glutathione peroxidase activity assay revealed that the BMS-Prdx VI would have the antioxidative activity in the skin.According to the data of the Bombina Maxima transcriptome, the full cDNA sequence of the BMS –Prdx VI and muture peptide of BMS –Prdx I and BMS –Prdx V have been cloned. Following design of the primer of BMS Prdx VI for the qRT-PCR, the distribution of the protein in eight tissues and the relative amount have been assayed. The amount of BMS Prdx VI in skin is five times more than that in other seven tissues and this suggests that BMS Prdx VI may play some physical functions in the skin.
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