Effects of Dimethyl Sulfoxide, Ethylene Glycol, Propylene Glycol and Glycerol on Cryopreservation of Wild Tree Shrew (Tupaia belangeri Chinese) Cauda Epididymal Sperm

KIZ OpenIR > 生殖与发育生物学

	Effects of Dimethyl Sulfoxide, Ethylene Glycol, Propylene Glycol and Glycerol on Cryopreservation of Wild Tree Shrew (Tupaia belangeri Chinese) Cauda Epididymal Sperm
	Ping SH 1; Yue F 2; Wang CY 1; Luo Y 1; Si W 3; Yang SH*1
	2012
发表期刊	JOURNAL OF ANIMAL AND VETERINARY ADVANCES
卷号	11 期号:19 页码:3568-3574
合作性质	其它
摘要	The objective was to examine the effect of Permeable Cryoprotectant Agents (CPAs) on sperm cryopreservation of tree shrew. Epididymal sperm were surgically harvested from captured wild male tree shrews and cryopreserved with Tes-Tris-Egg yolk based cryodiluent (TTE) containing either of the four CPAs, Dimethyl Sulfoxide (DMSO), Ethylene Glycol (EG), Propylene Glycol (PG) and Glycerol (Gly) at concentrations of 1, 3, 6 and 10%, respectively. Sperm motility, acrosome integrity and fertility were assessed. In Experiment 1, sperm equilibrated at 4 C in TTE containing 1, 3 and 6% DMSO, respectively showed similar motility to that in TTE without CPA (p>0.05). Following the increase of concentration of CPAs and equilibration time (30-90 min), the other CPAs reduced sperm motility (p<0.05). In Experiment 2, sperm frozen in TTE containing 3% DMSO showed the highest post-thaw motility (p<0.05) and recovery rate of motility (p<0.05) among groups. In Experiment 3, there were no differences in the fertilization rate of oocytes and the proportion of tree shrews yielding fertilized oocytes inseminated with fresh and thawed sperm frozen in TTE containing 3% DMSO (p>0.05). In conclusion, among the permeable CPAs tested, DMSO provided the best cryoprotective ability for captured wild tree shrew epididymal sperm.
关键词	Tree Shrew Sperm Cryopreservation Cryoprotectant Dmso China
收录类别	SCI
语种	英语
WOS记录号	WOS:000315851100018
引用统计
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/7293
专题	生殖与发育生物学中国科学院昆明灵长类研究中心
作者单位	1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Lab Mol Genet Aging & Tumor, Kunming 650093, Peoples R China 2.Capital Univ Med Sci, Xuanwu Hosp, Minist Educ,Beijing Inst Geriatr, Dept Neurobiol,Key Lab Neurodegenerat Dis, Beijing 100053, Peoples R China 3.Chinese Acad Sci, Kunming Inst Zool, Kunming Primate Res Ctr, Yunnan Key Lab Anim Reprod, Kunming 650223, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Ping SH,Yue F,Wang CY,et al. Effects of Dimethyl Sulfoxide, Ethylene Glycol, Propylene Glycol and Glycerol on Cryopreservation of Wild Tree Shrew (Tupaia belangeri Chinese) Cauda Epididymal Sperm[J]. JOURNAL OF ANIMAL AND VETERINARY ADVANCES,2012,11(19):3568-3574.
APA	Ping SH,Yue F,Wang CY,Luo Y,Si W,&Yang SH*.(2012).Effects of Dimethyl Sulfoxide, Ethylene Glycol, Propylene Glycol and Glycerol on Cryopreservation of Wild Tree Shrew (Tupaia belangeri Chinese) Cauda Epididymal Sperm.JOURNAL OF ANIMAL AND VETERINARY ADVANCES,11(19),3568-3574.
MLA	Ping SH,et al."Effects of Dimethyl Sulfoxide, Ethylene Glycol, Propylene Glycol and Glycerol on Cryopreservation of Wild Tree Shrew (Tupaia belangeri Chinese) Cauda Epididymal Sperm".JOURNAL OF ANIMAL AND VETERINARY ADVANCES 11.19(2012):3568-3574.

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