Discovery of Inhibitors To Block Interactions of HIV-1 Integrase with Human LEDGF/p75 via Structure-Based Virtual Screening and Bioassays

	Discovery of Inhibitors To Block Interactions of HIV-1 Integrase with Human LEDGF/p75 via Structure-Based Virtual Screening and Bioassays
	Hu GP 1; Li X1 ; Zhang X 2; Li YZ 1; Ma L1 ; Yang LM 2; Liu GX 1; Li WH 1; Huang J1; Shen X 3; Hu LH3; Zheng YT2; Tang Y1; huangjin@ecust.edu.cn ; simmhulh@mail.shcnc.ac.cn ; zhengyt@mail.kiz.ac.cn ; ytang234@ecust.edu.cn
	2012
发表期刊	JOURNAL OF MEDICINAL CHEMISTRY
卷号	55 期号:22 页码:10108-10117
摘要	This study aims to identify inhibitors that bind at the interface of HIV-1 integrase (IN) and human LEDGF/p75, which represents a novel target for anti-HIV therapy. To date, only a few such inhibitors have been reported. Here structure-based virtual screening was performed to search for the inhibitors from an in-house library of natural products and their derivatives. Among the 38 compounds selected by our strategy, 18 hits were discovered. The two most potent inhibitors showed IC50 values at 0.32 and 0.26 mu M, respectively. Three compounds were subsequently selected for anti-HIV assays, among which (E)-3-(2-chlorophenyl)-1-(2,4-dihydroxyphenyl)prop-2-en-1-one (NPD170) showed the highest antiviral activity (EC50 = 1.81 mu M). The antiviral mechanism of these compounds was further explored, and the results validated that the compounds interrupted the binding of transfected IN to endogenous LEDGF/p75. These findings could be helpful for anti-HIV drug discovery.
资助者	This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium).
收录类别	SCI
语种	英语
资助者	This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium). ; This work was supported by the Program for New Century Excellent Talents in University (Grant NCET-08-0774), the Fundamental Research Funds for the Central Universities (Grant WY1113007), the Shanghai Committee of Science and Technology (Grant 11DZ2260600), the National Natural Science Foundation of China (grants 30925040 and 81102483), and the Key Scientific and Technological Program of China (grants 2012ZX10001-006 and 2012ZX09103-101- 022). The cDNA coding for HIV IN catalytic core domain (residues 50−212) including the F185K solubilizing-mutation was a gift from Prof. Robert Craigie (National Institutes of Health, Bethesda, MD). The full-length plasmid pCPNat p75 was kindly provided by Prof. Zeger Debyser (Katholieke Universiteit Leuven, Belgium).
WOS记录号	WOS:000311461500057
引用统计
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/7304
专题	科研部门_分子免疫药理学（郑永唐）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	huangjin@ecust.edu.cn; simmhulh@mail.shcnc.ac.cn; zhengyt@mail.kiz.ac.cn; ytang234@ecust.edu.cn
作者单位	1.Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China 2.Key Laboratory of Animal Models and Human Diseases Mechanisms of Chinese Academy of Sciences and Yunnan, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.Shanghai Research Center for the Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
推荐引用方式 GB/T 7714	Hu GP,Li X,Zhang X,et al. Discovery of Inhibitors To Block Interactions of HIV-1 Integrase with Human LEDGF/p75 via Structure-Based Virtual Screening and Bioassays[J]. JOURNAL OF MEDICINAL CHEMISTRY,2012,55(22):10108-10117.
APA	Hu GP.,Li X.,Zhang X.,Li YZ.,Ma L.,...&ytang234@ecust.edu.cn.(2012).Discovery of Inhibitors To Block Interactions of HIV-1 Integrase with Human LEDGF/p75 via Structure-Based Virtual Screening and Bioassays.JOURNAL OF MEDICINAL CHEMISTRY,55(22),10108-10117.
MLA	Hu GP,et al."Discovery of Inhibitors To Block Interactions of HIV-1 Integrase with Human LEDGF/p75 via Structure-Based Virtual Screening and Bioassays".JOURNAL OF MEDICINAL CHEMISTRY 55.22(2012):10108-10117.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
BJ01275367.pdf（1292KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	请求全文