So far the knowledge on the early neural development in primates is not well understood due to the restrictions of ethics and material sources. Rhesus monkey embryonic stem cells (rESCs) are ideal candidates for basic research and generation of donor cells for transplantation therapies because rhesus monkey takes advantages over rodents in close genetic and physiological relation to human. This study mainly foucused on the differentiation of glial precursors (GPs) from Rhesus monkey embryonic stem cells (rESCs) and the mechanism regulating the migration of glial precursors (GPs). The results were shown as follows:1. Purified A2B5+ GPs were differentiated from rESCs via serum-free differentiation system. 2. NO (released from 10μM—250μM SNP) regulated the migration of the GPs A2B5+/ Nestin- GPs derived from rESCs in vitro. And extracellualr matrix (ECM) and integrin α6, integrin β1 participate this progress. 3. CO (released from 6-14 μg/ml Hematin) regulated the migration of the A2B5+/ Nestin- GPs derived from rESCs in vitro. And extracellualr matrix (ECM) and integrin α6, integrin β1 also participate this progress. Hereby, all of the results in this study would provide stage-specific donor cells for therapy on degenerative diseases in the CNS and established that small gas signal moleculer CO regulate the glial precursors migration in vitro. It has the implication for the glioblastoma therapy.
修改评论