This thesis is divided into two parts. The first part is the full-length amplification of two interleukin molecules of Bombina maxima. The second part is the polyclonal antibody preparation of caspase-1 in Bombina maxima. The first part, we amplified the cDNA ends of IL-8 and two IL-18 molecules of Bombina maxima using rapid amplification of cDNA ends (RACE). Interleukins are a type of cytokines commonly found in vertebrate involved in the resistance to routine microbial flora, modulating the immune response and mediating inflammatory response. They play a very important role in maintaining a homeostatic balance and the health of the body. First, we extracted RNA from Bombina maxima organizations using molecular biology means, then connected an adaptor to the end of the RNA, at the same time, reverse transcript it. Finally, using the known fragment specific primer and the adaptor complementary primer to PCR, the full-length of a chain can be obtained. Through experiments, we got the full-length of IL-8 and IL-18 coding region of Bombina maxima. And compared the resulting sequences and with other species. The findings in this part supplied sequence data for future immunological studies of Bombina maxima. The second part, we prepared polyclonal antibody of Bombina maxima caspase-1(that is rabbit antiserum). Caspases is named as cysteine-aspartic proteases or cysteine-dependent aspartate-directed proteases. It is a cysteine proteases family which plays a very important role in apoptosis, necrosis and inflammation. Caspase-1 is an enzyme related to inflammation that can be activated in the process of the formation of a inflammasome. Activated Caspase-1 can activate an IL-1beta or IL-18, in which way to initiate the immune response and involve in the immune response. For using of the E.coli expression system we first got Caspase-1 recombinant protein which then be used as an antigen to stimulate the New Zealand rabbit for rabbit antiserum. We did qualitative and quantitative analysis for the obtained antiserum by means of biochemistry. Finally, we got high-specific and high titer polyclona antibody. The preparation of the antibody provided experimental materials for the study of Bombina maxima inflammasome and a basis for further study of the form and function of the inflammasome.
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