Different Expression Profiles of Bioactive Peptides in Pelophylax nigromaculatus from Distinct Regions

	Different Expression Profiles of Bioactive Peptides in Pelophylax nigromaculatus from Distinct Regions
	Song YZ1; Ji SL 1; Liu W 1; Yu XY 1; Meng QX 1; Lai R2,3; uzhusong@kmust.edu.cn ; rlai@mail.kiz.ac.cn
	2013
发表期刊	BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷号	77 期号:5 页码:1075-1079
合作性质	其它
摘要	Amphibian skin is an abundant repository of bioactive peptides, important components of the defensive system. The variability of the bioactive peptide repertoires of individual species remains unclear. In this study, dark-spotted frogs were collected from Kunming in Yunnan Province, China and Guiyang in Guizhou Province, China to determine whether the bioactive peptides in amphibian skin differ between the two regions. Eight-antimicrobial peptides and an antioxidant peptide were identified by screening of cDNA library. Among the identified peptides, three antimicrobial peptides (pelophylaxin-2GY, temporin-1GY, and temporin-1KM) and an antioxidant peptide (antioxidin-PN) are reported here for the first time. Nigrocin-1, nigrocin-2, and pelophylaxin-2 were expressed by frogs in both regions. Pelophylaxin-2GY and temporin-1GY were found only in the frogs from Guiyang, whereas antioxidin-PN, esculetin-1, esculetin-2, and temporin-1KM were found only in those from Kunming. This difference was confirmed by allele-specific RT-PCR. The bioactive peptides expressed clearly varied between these populations of the same species.
关键词	Amphibian Skin Antimicrobial Peptides Antioxidant Peptide Different Expression Cdna Screening
收录类别	SCI
语种	英语
WOS记录号	WOS:000320414900029
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/7581
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	uzhusong@kmust.edu.cn; rlai@mail.kiz.ac.cn
作者单位	1.Engineering Research Center for Molecular Diagnosis, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnam, China 2.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Biotoxin Units, Kunming 650223, Yunnan, Peoples R China 3.Nanjing Agr Univ, Life Sci Coll, Nanjing 210095, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Song YZ*,Ji SL,Liu W,et al. Different Expression Profiles of Bioactive Peptides in Pelophylax nigromaculatus from Distinct Regions[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2013,77(5):1075-1079.
APA	Song YZ*.,Ji SL.,Liu W.,Yu XY.,Meng QX.,...&rlai@mail.kiz.ac.cn.(2013).Different Expression Profiles of Bioactive Peptides in Pelophylax nigromaculatus from Distinct Regions.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,77(5),1075-1079.
MLA	Song YZ*,et al."Different Expression Profiles of Bioactive Peptides in Pelophylax nigromaculatus from Distinct Regions".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 77.5(2013):1075-1079.

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