Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium

	Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium
	Zeng Y 1; Rong MQ 2; Liu YS 1; Liu JF 1; Lu M3; Tao XY 3; Li ZY 1; Chen X 1; Yang K 1; Li CT 1; Liu ZX1; lming163@yahoo.com.cn ; zhixiongliu@csu.edu.cn
	2013
发表期刊	NEUROCHEMICAL RESEARCH
卷号	38 期号:12 页码:2483-2489
合作性质	其它
摘要	Umbilical cord blood-derived marrow stromal cells (UCB-MSCs) with high proliferation capacity and immunomodulatory properties are considered to be a good candidate for cell-based therapies. But until now, little work has been focused on the differentiation of UCB-MSCs. In this work, UCB-MSCs were demonstrated to be negative for CD34 and CD45 expression but positive for CD90 and CD105 expression. The gate values of UCB-MSCs for CD90 and CD105 were 99.3 and 98.6 %, respectively. Two weeks after treatment, the percentage of neuron-like cells differentiated from UCB-MSCs was increased to 84 +/- A 12 % in the experimental group [treated with olfactory ensheathing cells (OECs)-conditioned medium] and they were neuron-specific enolase positive; few neuron-like cells were found in the control group (without OECs-conditioned medium). Using whole-cell recording, sodium and potassium currents were recorded in UCB-MSCs after differentiation by OECs. Thus, human UCB-MSCs could be differentiated to neural cells by secreted secretion from OECs and exhibited electrophysiological properties similar to mature neurons after 2 weeks post-induction. These results imply that OECs can be used as a new strategy for stem cell differentiation and provide an alternative neurogenesis pathway for generating sufficient numbers of neural cells for cell therapy.
关键词	Stem Cell Differentiation Olfactory Ensheathing Cells Ion Channel
收录类别	SCI
语种	英语
WOS记录号	WOS:000327131500004
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/7726
专题	科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	lming163@yahoo.com.cn; zhixiongliu@csu.edu.cn
作者单位	1.Department of Neurosurgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan Province 410008, China 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.Department of Neurosurgery, Second Affiliated Hospital of Hunan Normal University, 163
推荐引用方式 GB/T 7714	Zeng Y,Rong MQ,Liu YS,et al. Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium[J]. NEUROCHEMICAL RESEARCH,2013,38(12):2483-2489.
APA	Zeng Y.,Rong MQ.,Liu YS.,Liu JF.,Lu M*.,...&zhixiongliu@csu.edu.cn.(2013).Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium.NEUROCHEMICAL RESEARCH,38(12),2483-2489.
MLA	Zeng Y,et al."Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium".NEUROCHEMICAL RESEARCH 38.12(2013):2483-2489.

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