| 其他摘要 | Isolation and characterization of HIV-1 isolates in Yunnan province, China Ph.D candidate: Zhi-Hong Yao (Cell Biology) Directed by Prof. Yong-Tang Zheng （Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, graduate school of the Chinese Academy of Sciences,CAS） Acquired immunodeficiency syndrome (AIDS) caused by human immunodeficiency virus (HIV) is an important public health concern. The salient feature of this disease is that HIV specific attack helper T lymphocytes leading to immune system progressive damage and all sorts of opportunistic infections and related tumors. At present, there are one millions HIV-1 infected patients in China, and it is important to prevent and treat HIV infection effectively. A number of research results showed that Yunnan province is the origin site of China's major epidemic strains. Thus, comprehensive, systems, and ongoing HIV-1 molecular epidemiological studies in Yunnan province will play an important role in making an effective prevention strategy to preventing the HIV-1 prevalence in Yunnan province and even the national and developing effective Anti-HIV drugs and vaccines. The main purpose of this study was to improve and expand the HIV-1 reservoir from different areas of Yunnan province. And we hope to establish the virus seed bank of HIV-1 in Yunnan province including plasma, nucleic acid and HIV-1 primary isolates. Based on the research of HIV-1 biological characteristics, we hope to better grasp the AIDS epidemic characteristics in Yunnan province, investigate the source of transmission and the variation trend of HIV-1, which will provide a scientific basis for AIDS prevention and control measures, for the rational use of antiviral drugs on medicine. Setting up viral seed bank will play an important role in developing Anti-HIV drugs and vaccines and providing important information for research. In this study, a total of 3247 questionnaires and 259 whole bloods were collected in Kunming. A total of 2098 whole bloods from IUDs were recruited, with 480 were from Ying jiang, 474 from Baoshan, 329 from Kaiyuan, 581 from Zhaotong, 234 from Qujing. 3247 participants recruited from Kunming were HIV-1 positive patients. 85.2% of the participants aged from 18 to 50, suggested that sexual activity are more susceptible to infection. And among the participants, 94.46% (3067/3247) of them infected HIV-1 through heterosexual contact, 2.87% (93/3247) are intravenous drug use (IDUs), 1.35% (44/3247) through MSM and 0.83% (27/3247) through mother-to-child transmission (MTCT). In our study, we tested the infection status of HIV, HBV and HCV of 2080 intravenous drug users from different regions of Yunnan province using virus detection kit. The results showed that the infection rates among all participants were 25.5% for HIV, 77.7% for HCV, 19.2% for HBV, 15% for HIV/HCV, 0.3% for HIV/HBV, 7.8% for HCV/HBV and 7.1% for HIV/HBV/HCV. HCV is more prevalent than HIV and HBV among all IDUs. The prevalence of virus infection varied widely by regions in Yunnan province of China. It was notable that Baoshan not only had the lowest HIV infection, but also HBV and HCV infection (70.3% and 7.4% respectively). However, Kaiyuan had the highest ratio of HCV (89.1%), while Yingjiang was the most prevalent region of HBV (44/2%) among 5 sites. This study was carried out to isolate and study the biological characteristics of HIV-1 isolates in Yunnan province, China. A total of 421 HIV-1 clinical isolates was successfully cultured from 642 antiretroviral (ART)-na?ve HIV-1-positive individuals recruited from different regions of Yunnan province performed by co-culture of PBMCs/whole blood of HIV-positive patients with PBMCs of HIV-negative individuals. The separation rate was 65.57%. Among them, 293 strains were cultured by the method of Micro-Whole blood, the separation rate was 77.3%, and 128 strains were cultured by the method of PBMC trained, the separation rate was 49.4%. |
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