Flow cytometric characterizations of leukocyte subpopulations in the peripheral blood of northern pig-tailed macaques (Macaca leonina)

	Flow cytometric characterizations of leukocyte subpopulations in the peripheral blood of northern pig-tailed macaques (Macaca leonina)
	Zheng HY 1,2; Zhang MX 1,3; Zhang LT 1,4; Zhang XL 1,4; Pang W 1; Lv LB 1,5; Zheng YT*1,2,3,5; zhengyt@mail.kiz.ac.cn
	2014
发表期刊	Zoological Research
卷号	35 期号:6 页码:465-473
合作性质	其它
摘要	Pig-tailed macaques (Macaca nemistrina group) have been extensively used as non-human primate animal models for various human diseases in recent years, notably for AIDS research due to their sensitivity to HIV-1. Northern pig-tailed macaques (M. leonina) are distributed in China and other surrounding Southeast Asia countries. Although northern pig-tailed macaques have been bred on a large scale as experimental animals since 2012, the reference value of normal levels of leukocytes is not available. To obtain such information, 62 blood samples from male and female healthy northern pig-tailed macaques at different ages were collected. The normal range of major leukocyte subpopulations, such as T lymphocytes, B lymphocytes, natural killer (NK) cells, monocytes, and the expression levels of activation or differentiation related molecules (CD38, HLA-DR, CCR5, CD21, IgD, CD80 and CD86) on lymphocytes were analyzed by flow cytometry. The counts of B cells decreased with age, but those of CD8⁺ T cells and NK cells and the frequency of CD38⁺HLA-DR⁺CD4⁺ T cells were positively correlated with age. The counts of leukocyte subpopulations were higher in males than those in females except for CD4⁺ T cells. Males also showed higher expression levels of IgD and CD21 within B cells. This study provides basic data about the leukocyte subpopulations of northern pig-tailed macaques and compares this species with commonly used Chinese rhesus macaques (M. mulatta), which is meaningful for the biomedical application of northern pig-tailed macaques.
关键词	Northern Pig-tailed Macaque Flow Cytometry Leukocyte Subpopulation Age Sex
收录类别	其他
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/8170
专题	科研部门_分子免疫药理学（郑永唐）科研部门_动物模型与人类重大疾病机理重点实验室中国科学院昆明灵长类研究中心
通讯作者	zhengyt@mail.kiz.ac.cn
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China 2.School of Life Sciences, University of Science and Technology of China, Hefei Anhui 230026, China 3.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming Yunnan 650204, China 4.Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, China 5.Kunming Primate Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China
推荐引用方式 GB/T 7714	Zheng HY,Zhang MX,Zhang LT,et al. Flow cytometric characterizations of leukocyte subpopulations in the peripheral blood of northern pig-tailed macaques (Macaca leonina)[J]. Zoological Research,2014,35(6):465-473.
APA	Zheng HY.,Zhang MX.,Zhang LT.,Zhang XL.,Pang W.,...&zhengyt@mail.kiz.ac.cn.(2014).Flow cytometric characterizations of leukocyte subpopulations in the peripheral blood of northern pig-tailed macaques (Macaca leonina).Zoological Research,35(6),465-473.
MLA	Zheng HY,et al."Flow cytometric characterizations of leukocyte subpopulations in the peripheral blood of northern pig-tailed macaques (Macaca leonina)".Zoological Research 35.6(2014):465-473.

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