Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews

	Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews
	Li Gui 1; Lai R*1,2; Duan G 3; Lv LB 1; Zhang ZY 1; Liu H 1; Xiang X 3; rlai@mail.kiz.ac.cn
	2014
发表期刊	Zoological Research
卷号	35 期号:6 页码:492-499
合作性质	其它
摘要	Endosymbionts influence many aspects of their hosts' health conditions, including physiology, development, immunity, metabolism, etc. Tree shrews (Tupaia belangeri chinensis) have attracted increasing attention in modeling human diseases and therapeutic responses due to their close relationship with primates. To clarify the situation of symbiotic bacteria from their body surface, oral cavity, and anus, 12 wild and 12 the third generation of captive tree shrews were examined. Based on morphological and cultural characteristics, physiological and biochemical tests, as well as the 16S rDNA full sequence analysis, 12 bacteria strains were isolated and identified from the wild tree shrews: body surface: Bacillus subtilis (detection rate 42%),Pseudomonas aeruginosa (25%), Staphlococcus aureus (33%), S. Epidermidis (75%), Micrococcus luteus(25%), Kurthia gibsonii (17%); oral cavity: Neisseria mucosa (58%), Streptococcus pneumonia (17%); anus: Enterococcus faecalis (17%), Lactococus lactis (33%), Escherichia coli (92%), Salmonella typhosa(17%); whereas, four were indentified from the third generation captive tree shrews: body surface: S. epidermidis (75%); oral cavity: N.mucosa (67%); anus: L. lactis (33%), E. coli (100%). These results indicate that S. epidermidis, N. mucosa, L. lactis and E. coli were major bacteria in tree shrews, whereas,S. aureus, M. luteus, K. gibsonii, E. faecalis and S. typhosa were species-specific flora. This study facilitates the future use of tree shrews as a standard experimental animal and improves our understanding of the relationship between endosymbionts and their hosts.
关键词	Tree Shrew Microbial Separation Identification
收录类别	其他
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/8171
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室中国科学院昆明灵长类研究中心
通讯作者	rlai@mail.kiz.ac.cn
作者单位	1.Kunming Primate Research Center of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China 3.Yunnan Agricultural University, Kunming Yunnan 650201, China
推荐引用方式 GB/T 7714	Li Gui,Lai R*,Duan G,et al. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews[J]. Zoological Research,2014,35(6):492-499.
APA	Li Gui.,Lai R*.,Duan G.,Lv LB.,Zhang ZY.,...&rlai@mail.kiz.ac.cn.(2014).Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews.Zoological Research,35(6),492-499.
MLA	Li Gui,et al."Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews".Zoological Research 35.6(2014):492-499.

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