| Rapid analysis for the identification of the seagrass Halophila ovalis (Hydrocharitaceae) | |
| Osathanunkul M*1; Suwannapoom C2; Singtonat S1; Poomipoo N1; Jampeetong A1; Madesis P3 | |
| 2015 | |
| 发表期刊 | African Journal of Biotechnology
 |
| 卷号 | 14期号:8页码:649-656 |
| 摘要 | Seagrasses are considered as one of the most important species as they play key ecological roles in various types of ecosystems and also provide a food source for endangered animal species. There are two main characteristics of seagrasses that hinder efforts to correctly identify species based on conventional identification keys alone: i) the variability of morphological characteristics and ii) lack of needed morphological characters especially flowers. A taxonomically unresolved complex such as Halophila spp. is reported. Plant DNA barcoding regions (rbcL and trnH-psbA) were used to confirm species of collected seagrasses from the southern coast of Thailand. Small and big-leaved samples of Halophila spp. were analysed in this study. The big-leaved samples were identified on the field as Halophila ovalis whilst it was uncertain whether the small-leaved samples belonged to H. ovalis. DNA analysis revealed that the small-leaved samples could be H. ovalis. We also coupled PCR with high resolution melt (HRM) to more cost-effectively identify individuals of H. ovilis than using barcoding alone. Using HRM to resolve differences in the sequence of two genes showed that the two unknown seagrasses belonged to the same species as H. ovalis. In conclusion, using HRM proved to pose great potential in seagrass identification. |
| 关键词 | Dna Barcoding Halophila Ovalis. Rbcl Trnh-psba Species Identification |
| 收录类别 | 其他 |
| 语种 | 英语 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/152453/9355 |
| 专题 | 遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 650223,China 3.Institute of Applied Biosciences, Centre for Research and Technology Hellas (CERTH), Thessaloniki, Greece |
| 推荐引用方式 GB/T 7714 | Osathanunkul M*,Suwannapoom C,Singtonat S,et al. Rapid analysis for the identification of the seagrass Halophila ovalis (Hydrocharitaceae)[J]. African Journal of Biotechnology,2015,14(8):649-656. |
| APA | Osathanunkul M*,Suwannapoom C,Singtonat S,Poomipoo N,Jampeetong A,&Madesis P.(2015).Rapid analysis for the identification of the seagrass Halophila ovalis (Hydrocharitaceae).African Journal of Biotechnology,14(8),649-656. |
| MLA | Osathanunkul M*,et al."Rapid analysis for the identification of the seagrass Halophila ovalis (Hydrocharitaceae)".African Journal of Biotechnology 14.8(2015):649-656. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A2015091007.pdf（910KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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