Purification and cmiucterization of alpha-mucrofibrase, a novel serine protease with alpha-fibrinogenase activity from the venom of Chinese Habu (Trimeresurus mucrosquamatus)

	Purification and cmiucterization of alpha-mucrofibrase, a novel serine protease with alpha-fibrinogenase activity from the venom of Chinese Habu (Trimeresurus mucrosquamatus)
	Wei Q 1,2; Jin Y 1,2; Xiong YL*1; Lu QM 1; Wei JF 1; Wang WY 1; weiqinwq@yahoo.com.cn
	2002
发表期刊	JOURNAL OF NATURAL TOXINS
卷号	11 期号:4 页码:337-343
合作性质	其它
摘要	A novel fibrinogenolytic protease, named alpha-mucrofibrase, was purified from the venom of Chinese Habu (Trimeresurus mucrosquamatus) by DEAE-Sephadex A-50 ion-exchange chromatography and Sephadex G-100 (super fine) gel filtration alpha-Mucrofibrase is a single-chain polypeptide of approximately 29 kDa. It is stable even at 95degreesC, and the most susceptible hydrolysis substrate is S-2302. It cleaved primarily the Aalpha chain of fibrinogen followed by the Bbeta chain, while the gamma chain was partially affected. N-terminal sequence of this fibrinogenolytic enzyme has great homology with those of other snake venom serine proteases. The esterase activity of alpha-mucrofibrase is inhibited by phenylmethylsulfonyl fluoride (PMSF) but not by metal chelator (EDTA), suggesting this fibrinogenase belongs to the venom serine protease family.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/9680
专题	科研部门_天然药物功能蛋白质学科组（赖仞）其他
通讯作者	weiqinwq@yahoo.com.cn
作者单位	1.Chinese Acad Sci, Kunming Inst Zool, Dept Anim Toxinol, Kunming 650223, Peoples R China 2.Chinese Acad Sci, Grad Sch, Kunming 650223, Peoples R China
推荐引用方式 GB/T 7714	Wei Q,Jin Y,Xiong YL*,et al. Purification and cmiucterization of alpha-mucrofibrase, a novel serine protease with alpha-fibrinogenase activity from the venom of Chinese Habu (Trimeresurus mucrosquamatus)[J]. JOURNAL OF NATURAL TOXINS,2002,11(4):337-343.
APA	Wei Q.,Jin Y.,Xiong YL*.,Lu QM.,Wei JF.,...&weiqinwq@yahoo.com.cn.(2002).Purification and cmiucterization of alpha-mucrofibrase, a novel serine protease with alpha-fibrinogenase activity from the venom of Chinese Habu (Trimeresurus mucrosquamatus).JOURNAL OF NATURAL TOXINS,11(4),337-343.
MLA	Wei Q,et al."Purification and cmiucterization of alpha-mucrofibrase, a novel serine protease with alpha-fibrinogenase activity from the venom of Chinese Habu (Trimeresurus mucrosquamatus)".JOURNAL OF NATURAL TOXINS 11.4(2002):337-343.

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