L-amino acid oxidase from Trimeresurus jerdonii snake venom: Purification, characterization, platelet aggregation-inducing and antibacterial effects

	L-amino acid oxidase from Trimeresurus jerdonii snake venom: Purification, characterization, platelet aggregation-inducing and antibacterial effects
	Lu QM; Wei Q; Jin Y; Wei JF; Wang WY; Xiong YL*; wangwy@mail.kiz.ac.cn
	2002
发表期刊	JOURNAL OF NATURAL TOXINS
卷号	11 期号:4 页码:345-352
合作性质	其它
摘要	An L-amino acid oxidase (LAO), designated as TJ-LAO, was purified to homogeneity from the venom of Trimeresurus jerdonii by Sephadex G-100 and Q Sepharose HP chromatography. The molecular weight of this enzyme was 110 kD as estimated by analytical gel filtration and was 55 kD by SDS-polyacrylamide gel electrophoresis, suggesting that the enzyme is composed of two subunits. ne enzyme has an absorption spectrum characteristic of flavoproteins, containing 2 moles of FMN per mole of enzyme. The N-terminal sequence of TJ-LAO shares high homology with other viperid snake venom LAOs. Homology with elapid venom LAO is lower. TJ-LAO inhibited the growth of Escherichia coli. Staphylococcus aureus Pseudomonas aeruginosa, and Bacillus megaterium. The antibacterial effect associated with LAO activity was elminated with the addition of catalase. Platelets in platelet-rich plasma aggregated upon the addition of TJ-LAO. The enzyme-induced aggregation was inhibited by catalase, suggesting formation of H-2 O-2 was essential for TJ-LAO to induce platelet aggregation. These results showed H-2 O-2 formation is important for the biological effects of LAO.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/152453/9681
专题	科研部门_天然药物功能蛋白质学科组（赖仞）其他
通讯作者	wangwy@mail.kiz.ac.cn
作者单位	Chinese Acad Sci, Kunming Inst Zool, Dept Anim Toxinol, Kunming 650223, Peoples R China
推荐引用方式 GB/T 7714	Lu QM,Wei Q,Jin Y,et al. L-amino acid oxidase from Trimeresurus jerdonii snake venom: Purification, characterization, platelet aggregation-inducing and antibacterial effects[J]. JOURNAL OF NATURAL TOXINS,2002,11(4):345-352.
APA	Lu QM.,Wei Q.,Jin Y.,Wei JF.,Wang WY.,...&wangwy@mail.kiz.ac.cn.(2002).L-amino acid oxidase from Trimeresurus jerdonii snake venom: Purification, characterization, platelet aggregation-inducing and antibacterial effects.JOURNAL OF NATURAL TOXINS,11(4),345-352.
MLA	Lu QM,et al."L-amino acid oxidase from Trimeresurus jerdonii snake venom: Purification, characterization, platelet aggregation-inducing and antibacterial effects".JOURNAL OF NATURAL TOXINS 11.4(2002):345-352.

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