| Species authentication of commercial beef jerky based on PCR-RFLP analysis of the mitochondrial 12S rRNA gene | |
| Chen SY1,2; Liu YP2; Yao YG[*]1; ygyaozh@gmail.com; yaoyg@mail.kiz.ac.cn | |
| 2010 | |
| 发表期刊 | JOURNAL OF GENETICS AND GENOMICS
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| 卷号 | 37期号:11页码:763-769 |
| 合作性质 | 其它 |
| 摘要 | In this study, we determined species-specific variations by analyzing the mitochondrial 12S rRNA gene sequence variation (similar to 440 bp) in 17 newly obtained sequences and 90 published cattle, yak, buffalo, goat, and pig sequences, which represent 62 breeds and 17 geographic regions. Based on the defined species-specific variations, two endonucleases, Alu I and Bfa I, were selected for species authentication using raw meat/tissue samples and the PCR-RFLP method. Goat and pig were identified using the Alu I enzyme, while cattle, yak, and buffalo were identified by digestion with Bfa I. Our approach had relatively high detection sensitivity of cattle DNA in mixed cattle and yak products, with the lowest detectable threshold equaling 20% of cattle DNA in a mixed cattle/yak sample. This method was successfully used to type commercial beef jerky products, which were produced by different companies utilizing various processing technologies. Our results show that several yak jerky products might be implicated in commercial fraud by using cattle meat instead of yak meat. |
| 关键词 | 12s Rrna Gene Pcr-rflp Meat Species Identification Beef Jerky Commercial Fraud |
| 资助者 | This work was supported by the National High-tech R&D Program (863 Program) (No. 2008AA101001), the Ministry of Agriculture of China (No. 2009ZX08009- 159B), and Yunnan Province (No. 2009CI119). ; This work was supported by the National High-tech R&D Program (863 Program) (No. 2008AA101001), the Ministry of Agriculture of China (No. 2009ZX08009- 159B), and Yunnan Province (No. 2009CI119). ; This work was supported by the National High-tech R&D Program (863 Program) (No. 2008AA101001), the Ministry of Agriculture of China (No. 2009ZX08009- 159B), and Yunnan Province (No. 2009CI119). ; This work was supported by the National High-tech R&D Program (863 Program) (No. 2008AA101001), the Ministry of Agriculture of China (No. 2009ZX08009- 159B), and Yunnan Province (No. 2009CI119). |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助者 | This work was supported by the National High-tech R&D Program (863 Program) (No. 2008AA101001), the Ministry of Agriculture of China (No. 2009ZX08009- 159B), and Yunnan Province (No. 2009CI119). ; This work was supported by the National High-tech R&D Program (863 Program) (No. 2008AA101001), the Ministry of Agriculture of China (No. 2009ZX08009- 159B), and Yunnan Province (No. 2009CI119). ; This work was supported by the National High-tech R&D Program (863 Program) (No. 2008AA101001), the Ministry of Agriculture of China (No. 2009ZX08009- 159B), and Yunnan Province (No. 2009CI119). ; This work was supported by the National High-tech R&D Program (863 Program) (No. 2008AA101001), the Ministry of Agriculture of China (No. 2009ZX08009- 159B), and Yunnan Province (No. 2009CI119). |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kiz.ac.cn/handle/353002/6550 |
| 专题 | 科研部门_疾病机理遗传学和进化医学学科组（姚永刚） 科研部门_动物模型与人类重大疾病机理重点实验室 |
| 通讯作者 | ygyaozh@gmail.com; yaoyg@mail.kiz.ac.cn |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China 2.Research Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Ya’an 625014, China |
| 推荐引用方式 GB/T 7714 | Chen SY,Liu YP,Yao YG[*],et al. Species authentication of commercial beef jerky based on PCR-RFLP analysis of the mitochondrial 12S rRNA gene[J]. JOURNAL OF GENETICS AND GENOMICS,2010,37(11):763-769. |
| APA | Chen SY,Liu YP,Yao YG[*],ygyaozh@gmail.com,&yaoyg@mail.kiz.ac.cn.(2010).Species authentication of commercial beef jerky based on PCR-RFLP analysis of the mitochondrial 12S rRNA gene.JOURNAL OF GENETICS AND GENOMICS,37(11),763-769. |
| MLA | Chen SY,et al."Species authentication of commercial beef jerky based on PCR-RFLP analysis of the mitochondrial 12S rRNA gene".JOURNAL OF GENETICS AND GENOMICS 37.11(2010):763-769. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 2010122003.pdf（759KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 请求全文 | |
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