Purification and characterization of two new allergens from the salivary glands of the horsefly, Tabanusyao

	Purification and characterization of two new allergens from the salivary glands of the horsefly, Tabanusyao
	Ma D 1,2; Li Y 1,2; Dong J 3; An S 2,4; Wang Y 1,2; Liu C 1,2; Yang X 1,2; Yang H 1,2; Xu X 1; Lin D 3; Lai R*1; rlai@mail.kiz.ac.cn
	2011
发表期刊	ALLERGY
卷号	66 期号:1 页码:101-109
合作性质	其它
摘要	Background: Horsefly bite can cause allergic reactions in humans. There is no information about allergenic horsefly proteins. Objectives: The current work aims to purify and characterize IgE-binding proteins from horsefly salivary glands. Methods: Two IgE-binding proteins, Tab a 1 and Tab a 2 with molecular weight of 26 and 35 kd, respectively, were purified and characterized from 60 000 pairs of horsefly salivary glands of Tabanus yao, respectively. Their primary sequences were determined by Edman degradation and cDNA cloning. Their allergenicity was examined using enzyme-linked immunosorbent assay (ELISA), ELISA inhibition tests, and immunoblots. Results: Immunoblotting demonstrated IgE binding by 32 and 34 of 37 (86.5% and 91.8%) subjects' sera to Tab a 1 and Tab a 2, respectively. They were identified as an antigen 5-related (Ag 5) protein and hyaluronidase, respectively. ELISA inhibitions of serum IgE reactivity to the horsefly salivary gland extract (SGE) using purified Tab a 1 and Tab a 2 were significant (about 45%). In addition, these proteins showed some IgE-binding capacity to sera of subjects with wasp sting allergy. Conclusions: We have first identified and characterized two IgE-binding proteins, Tab a 1, an Ag 5-like protein and Tab a 2, a hyaluronidase, from the horsefly salivary glands. They appear to be of importance for the allergic reactions induced by horsefly bite. These allergens are thus not only found in stinging but also found in hematophagous insects. These results also provided support for the presence of the so-called wasp-horsefly syndrome (WHS).
关键词	Allergen Antigen 5 Hematophagous Horsefly Salivary Gland Hyaluronidase
资助者	This work was supported by NSFC (30830021) and MOST (2010CB529800). ; This work was supported by NSFC (30830021) and MOST (2010CB529800). ; This work was supported by NSFC (30830021) and MOST (2010CB529800). ; This work was supported by NSFC (30830021) and MOST (2010CB529800).
收录类别	SCI
语种	英语
资助者	This work was supported by NSFC (30830021) and MOST (2010CB529800). ; This work was supported by NSFC (30830021) and MOST (2010CB529800). ; This work was supported by NSFC (30830021) and MOST (2010CB529800). ; This work was supported by NSFC (30830021) and MOST (2010CB529800).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6556
专题	科研部门_天然药物功能蛋白质学科组（赖仞）
通讯作者	rlai@mail.kiz.ac.cn
作者单位	1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 2.Graduate School of the Chinese Academy of Sciences, Beijing, China 3.School of Life Science and Technology, China Pharmaceutical University, Nanjing, China 4.School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China
推荐引用方式 GB/T 7714	Ma D,Li Y,Dong J,et al. Purification and characterization of two new allergens from the salivary glands of the horsefly, Tabanusyao[J]. ALLERGY,2011,66(1):101-109.
APA	Ma D.,Li Y.,Dong J.,An S.,Wang Y.,...&rlai@mail.kiz.ac.cn.(2011).Purification and characterization of two new allergens from the salivary glands of the horsefly, Tabanusyao.ALLERGY,66(1),101-109.
MLA	Ma D,et al."Purification and characterization of two new allergens from the salivary glands of the horsefly, Tabanusyao".ALLERGY 66.1(2011):101-109.

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