A Young Drosophila Duplicate Gene Plays Essential Roles in Spermatogenesis by Regulating Several Y- Linked Male Fertility Genes

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	A Young Drosophila Duplicate Gene Plays Essential Roles in Spermatogenesis by Regulating Several Y- Linked Male Fertility Genes
	Ding Y 1,2; Zhao L 1,2; Yang S 1; Jiang Y 1,2; Chen Y 1,2; Zhao RP 1; Zhang Y 1; Zhang GJ 1,2; Dong Y 1,2; Yu HJ 3; Zhou Q 1,2; Wang W*1; wwang@mail.kiz.ac.cn
	2010
发表期刊	PLOS GENETICS
卷号	6 期号:12 页码:e1001255
合作性质	其它
摘要	Gene duplication is supposed to be the major source for genetic innovations. However, how a new duplicate gene acquires functions by integrating into a pathway and results in adaptively important phenotypes has remained largely unknown. Here, we investigated the biological roles and the underlying molecular mechanism of the young kep1 gene family in the Drosophila melanogaster species subgroup to understand the origin and evolution of new genes with new functions. Sequence and expression analysis demonstrates that one of the new duplicates, nsr (novel spermatogenesis regulator), exhibits positive selection signals and novel subcellular localization pattern. Targeted mutagenesis and whole-transcriptome sequencing analysis provide evidence that nsr is required for male reproduction associated with sperm individualization, coiling, and structural integrity of the sperm axoneme via regulation of several Y chromosome fertility genes post-transcriptionally. The absence of nsr-like expression pattern and the presence of the corresponding cis-regulatory elements of the parental gene kep1 in the pre-duplication species Drosophila yakuba indicate that kep1 might not be ancestrally required for male functions and that nsr possibly has experienced the neofunctionalization process, facilitated by changes of trans-regulatory repertories. These findings not only present a comprehensive picture about the evolution of a new duplicate gene but also show that recently originated duplicate genes can acquire multiple biological roles and establish novel functional pathways by regulating essential genes
资助者	This work was supported by a CAS key grant (KSCX2-YW-N-060), a NSFC key grant (No. 30930056), the 100 Talents Program of Chinese Academy of Sciences, and a CAS-Max Planck Society Fellowship to WW and by a NSFC grant (No. 30871342) and a grant (GREKF08-02) from the State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, to HY. ; This work was supported by a CAS key grant (KSCX2-YW-N-060), a NSFC key grant (No. 30930056), the 100 Talents Program of Chinese Academy of Sciences, and a CAS-Max Planck Society Fellowship to WW and by a NSFC grant (No. 30871342) and a grant (GREKF08-02) from the State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, to HY. ; This work was supported by a CAS key grant (KSCX2-YW-N-060), a NSFC key grant (No. 30930056), the 100 Talents Program of Chinese Academy of Sciences, and a CAS-Max Planck Society Fellowship to WW and by a NSFC grant (No. 30871342) and a grant (GREKF08-02) from the State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, to HY. ; This work was supported by a CAS key grant (KSCX2-YW-N-060), a NSFC key grant (No. 30930056), the 100 Talents Program of Chinese Academy of Sciences, and a CAS-Max Planck Society Fellowship to WW and by a NSFC grant (No. 30871342) and a grant (GREKF08-02) from the State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, to HY.
收录类别	SCI
语种	英语
资助者	This work was supported by a CAS key grant (KSCX2-YW-N-060), a NSFC key grant (No. 30930056), the 100 Talents Program of Chinese Academy of Sciences, and a CAS-Max Planck Society Fellowship to WW and by a NSFC grant (No. 30871342) and a grant (GREKF08-02) from the State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, to HY. ; This work was supported by a CAS key grant (KSCX2-YW-N-060), a NSFC key grant (No. 30930056), the 100 Talents Program of Chinese Academy of Sciences, and a CAS-Max Planck Society Fellowship to WW and by a NSFC grant (No. 30871342) and a grant (GREKF08-02) from the State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, to HY. ; This work was supported by a CAS key grant (KSCX2-YW-N-060), a NSFC key grant (No. 30930056), the 100 Talents Program of Chinese Academy of Sciences, and a CAS-Max Planck Society Fellowship to WW and by a NSFC grant (No. 30871342) and a grant (GREKF08-02) from the State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, to HY. ; This work was supported by a CAS key grant (KSCX2-YW-N-060), a NSFC key grant (No. 30930056), the 100 Talents Program of Chinese Academy of Sciences, and a CAS-Max Planck Society Fellowship to WW and by a NSFC grant (No. 30871342) and a grant (GREKF08-02) from the State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, to HY.
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6559
专题	基因起源组遗传资源与进化国家重点实验室
通讯作者	wwang@mail.kiz.ac.cn
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.Graduate University of Chinese Academy of Sciences, Beijing, China 3.Laboratory for Conservation and Utilization of Bio-Resources and Human Genetics Center of Yunnan University, Kunming, China
推荐引用方式 GB/T 7714	Ding Y,Zhao L,Yang S,et al. A Young Drosophila Duplicate Gene Plays Essential Roles in Spermatogenesis by Regulating Several Y- Linked Male Fertility Genes[J]. PLOS GENETICS,2010,6(12):e1001255.
APA	Ding Y.,Zhao L.,Yang S.,Jiang Y.,Chen Y.,...&wwang@mail.kiz.ac.cn.(2010).A Young Drosophila Duplicate Gene Plays Essential Roles in Spermatogenesis by Regulating Several Y- Linked Male Fertility Genes.PLOS GENETICS,6(12),e1001255.
MLA	Ding Y,et al."A Young Drosophila Duplicate Gene Plays Essential Roles in Spermatogenesis by Regulating Several Y- Linked Male Fertility Genes".PLOS GENETICS 6.12(2010):e1001255.

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