Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata

	Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata
	Liu XH 1; Liu R 1; Wei L 1; Yang HL 2; Zhang KY 1; Liu JZ3; Lai R1,2; jzliu21@heinfo.net ; rlai72@njau.edu.cn
	2011
发表期刊	JOURNAL OF PEPTIDE SCIENCE
卷号	17 期号:1 页码:68-72
合作性质	其它
摘要	Two novel antimicrobial peptides with similarity to brevinin-2 family are purified and characterized from the skin secretions of the frog, Rana nigrovittata. Their amino acid sequences were determined as GAFGNFLKGVAKKAGLKILSIAQCKLSGTC (brevinin-2-RN1) and GAFGNFLKGVAKKAGLKILSIAQCKLFGTC (brevinin-2-RN2), respectively, by Edman degradation. Different from brevinin-2, which is composed of 33 amino acid residues (aa), both brevinin-2-RN1 and -RN2 contain 30 aa. Five cDNA sequences (Genbank accession numbers, EU136465-9) encoding precursors of brevinin-2-RN1 and -RN2 were screened from the skin cDNA library of R. nigrovittata. These precursors are composed of 72 aa including a predicted signal peptide, an acidic spacer peptide, and a mature brevinin-2-RN. Both brevinin-2-RN1 and -RN2 showed strong antimicrobial activities against gram-positive and gram-negative bacteria and fungi. The current work identified and characterized two novel antimicrobial peptides with unique primary structure
关键词	Antimicrobial Peptides Amphibian Skin Brevinin-2 Rana Nigrovittata
资助者	This work was supported by the Chinese Academy of Sciences (KSCX2-YW-G-024), the Ministry of Science and Technology (2010CB529800), and the Ministry of Agriculture (2009ZX08009- 159B). ; This work was supported by the Chinese Academy of Sciences (KSCX2-YW-G-024), the Ministry of Science and Technology (2010CB529800), and the Ministry of Agriculture (2009ZX08009- 159B). ; This work was supported by the Chinese Academy of Sciences (KSCX2-YW-G-024), the Ministry of Science and Technology (2010CB529800), and the Ministry of Agriculture (2009ZX08009- 159B). ; This work was supported by the Chinese Academy of Sciences (KSCX2-YW-G-024), the Ministry of Science and Technology (2010CB529800), and the Ministry of Agriculture (2009ZX08009- 159B).
收录类别	SCI
语种	英语
资助者	This work was supported by the Chinese Academy of Sciences (KSCX2-YW-G-024), the Ministry of Science and Technology (2010CB529800), and the Ministry of Agriculture (2009ZX08009- 159B). ; This work was supported by the Chinese Academy of Sciences (KSCX2-YW-G-024), the Ministry of Science and Technology (2010CB529800), and the Ministry of Agriculture (2009ZX08009- 159B). ; This work was supported by the Chinese Academy of Sciences (KSCX2-YW-G-024), the Ministry of Science and Technology (2010CB529800), and the Ministry of Agriculture (2009ZX08009- 159B). ; This work was supported by the Chinese Academy of Sciences (KSCX2-YW-G-024), the Ministry of Science and Technology (2010CB529800), and the Ministry of Agriculture (2009ZX08009- 159B).
文献类型	期刊论文
条目标识符	http://ir.kiz.ac.cn/handle/353002/6562
专题	科研部门_天然药物功能蛋白质学科组（赖仞）科研部门_动物模型与人类重大疾病机理重点实验室
通讯作者	jzliu21@heinfo.net; rlai72@njau.edu.cn
作者单位	1.Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095,China 2.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming650223, Yunnan, China 3.CollegeofLifeSciencesSchoolofHebeiNormalUniversity,Shijiazhuang,Hebei 050016,China
推荐引用方式 GB/T 7714	Liu XH,Liu R,Wei L,et al. Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata[J]. JOURNAL OF PEPTIDE SCIENCE,2011,17(1):68-72.
APA	Liu XH.,Liu R.,Wei L.,Yang HL.,Zhang KY.,...&rlai72@njau.edu.cn.(2011).Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata.JOURNAL OF PEPTIDE SCIENCE,17(1),68-72.
MLA	Liu XH,et al."Two novel antimicrobial peptides from skin secretions of the frog, Rana nigrovittata".JOURNAL OF PEPTIDE SCIENCE 17.1(2011):68-72.

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